SRP3007

アンジオポエチン-1 ヒト

recombinant, expressed in HeLa cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• 別名: ANG-1, ANGTP1, KIAA003
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in HeLa cells
• アッセイ: ≥95% (HPLC); ≥95% (SDS-PAGE)
• 形状: lyophilized
• 分子量: 60.0-70.0 kDa
• 包装: pkg of 5 μg
• テクニック: cell culture | mammalian: suitable
• 不純物: <0.1 EU/μg endotoxin, tested
• 色: white to off-white
• UniProtアクセッション番号: Q15389
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... ANGPT1(284)

詳細

詳細

Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2, a tyrosine-kinase receptor expressed primarily on vascular endothelial cells and early hematopoietic cells. The gene encoding this protein is localized on human chromosome 8q23.1. Recombinant human ANG-1, derived from HeLa cells, is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0-70.0kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows N-terminal sequences starting with Ser-20 and with Asp-70 of the 498 amino acid precursor protein.

生物化学的／生理学的作用

Ang-1/Tie-2 signaling promotes angiogenesis during the development, remodeling, and repair of the vascular system. Transgenic mice lacking expression of either Ang-1 or Tie-2 fail to develop a fully functional cardiovascular system and die before birth. Postnatally, the angiogenic activity of Ang-1/Tie-2 is required during normal tissue repair and remodeling of the female endometrium in the menstrual cycle. Ang-1/Tie-2 signaling appears to be regulated by Angiopoietin-2 (Ang-2), a natural antagonist for Tie-2 that exerts its effects through an internal autocrine loop mechanism. In addition to suppressing endothelial cell activation by inhibiting the expression of adhesion and inflammatory molecules, Ang-1 enhances endothelial cell survival and capillary morphogenesis, and lessens capillary permeability. As such, Ang-1 has a potential to become an effective therapeutic agent for treating various endothelium disorders, including several severe human pulmonary diseases. The efficacy of cell-based Ang-1 gene therapy for acute lung injury (ALI) has recently been studied in a rat model of ALI. The results of this study show that such therapy can markedly improve lung condition and suggest that Ang-1 therapy may represent a potential new strategy for the treatment and/or prevention of acute respiratory distress injury (ARDI), a significant cause of morbidity and mortality in critically ill patients.

シーケンス

MSNQRRSPEN SGRRYNRIQH GQCAYTFILP EHDGNCREST TDQYNTNALQ RDAPHVEPDF SSQKLQHLEH MENYTQWLQ KLENYIVENM KSEMAQIQQN AVQNHTATML EIGTSLLSQT AEQTRKLTDV ETQVLNQTSR LEIQLLENSL STYKLEKQLL QQTNEILKIH EKNSLLEHKI LEMEGKHKEE LDTLKEEKEN LQGLVTRQTY IIQELEKQLN RATTNNSVLQ KQQLELMDTV HNLVNLCTKE GVLLKGGKRE EEKPFRDCAD VYQAGFNKSG IYTIYINNMP EPKKVFCNMD VNGGGWTVIQ HREDGSLDFQ RGWKEYKMGF GNPSGEYWLG NEFIFAITSQ RQYMLRIELM DWEGNRAYSQ YDRFHIGNEK QNYRLYLKGH TGTAGKQSSL ILHGADFSTK DADNDNCMCK CALMLTGGWW FDACGPSNLN GMFYTAGQNH GKLNGIKWHY FKGPSYSLRS TTMMIRPLDF

物理的形状

凍結乾燥品(20mM リン酸ナトリウム、pH 7.5.、200mM NaCl、5% トレハロース)

再構成

バイアルは、開封前に遠心してください。水で濃度1.0 mg/mLに復元してください。ボルテックスは使用しないでください。水溶液は2～8°Cで最長1週間保存できます。長期保存には、担体タンパク質(0.1% BSAなど)含有バッファーでさらに希釈し、1回の使用量ずつに分注して-20～-80°Cで保存することを推奨します。

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable