SRP0416

T4 Beta-glucosyltransferase

recombinant, expressed in E. coli, ≥83% (SDS-PAGE)

• 別名: T4-phage beta-glucosyltransferase, UDP glucose-DNA ?-glucosyltransferase, hydroxymethyl cytosine
• UNSPSCコード: 12352204
• NACRES: NA.32

特性

• 由来生物: Escherichia coli
• リコンビナント: expressed in E. coli
• アッセイ: ≥83% (SDS-PAGE)
• フォーム: aqueous solution
• 分子量: 41.6 kDa
• 包装: pkg of 100 μg
• NCBIアクセッション番号: NP_049658
• UniProtアクセッション番号: P04547
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: bacteriophage T4 ... B-GT(1258765)

詳細

詳細

T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.

アプリケーション

Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

生物化学的／生理学的作用

T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg²⁺, Mn²⁺ and Ca²⁺ activate this enzyme.[1][2][3]

物理的形状

Formulated in 200 mM imidazole and 20% glycerol.

安全性情報

• ピクトグラム: GHS08,GHS07
• シグナルワード: Danger
• 危険有害性情報: H315,H319,H360D
• 注意書き: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危険有害性の分類: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable