SRP0320

JMJD2b active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

• 別名: KDM4B, lysine (K)-specific demethylase 4B
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected Sf9 cells
• アッセイ: ≥70% (SDS-PAGE)
• フォーム: aqueous solution
• 分子量: 83 kDa
• 包装: pkg of 100 μg
• NCBIアクセッション番号: NM_015015
• UniProtアクセッション番号: O94953
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... KDM4B(23030)

詳細

詳細

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), amino acids 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system.

特異性

0.32 pmole/min/μg Assay conditions: 10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM a-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween20, 0.5 μM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin conjugated secondary antibody followed by Alpha Screening detection.

アプリケーション

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

物理的形状

Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol.

保管および安定性

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable