SRP0168

EED human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• 別名: HEED, Rinchik 5, WAIT1, WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected insect cells
• アッセイ: ≥90% (SDS-PAGE)
• 形状: aqueous solution
• 分子量: 51 kDa
• 包装: pkg of 50 μg
• 濃度: >0.02 mg/mL
• NCBIアクセッション番号: NM_003797
• UniProtアクセッション番号: O75530
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... EED(8726)

詳細

詳細

EED (ectoderm development protein) is the part of the polycomb repressive complex 2 (PRC2) where it interacts with EZH2 (enhancer of zeste homolog 2). The protein has seven WD domains (β-transducin domains). The EED gene is mapped to human chromosome 11q14.
Human Embryonic Ectoderm Development (hEED), also known as WAIT-1, GenBank Accession No. NM_003797, amino acids 2-end with N-terminal FLAG-tag, MW = 51kDa, expressed in Sf9 cells via a baculovirus expression system.

生物化学的／生理学的作用

EED (ectoderm development protein) is required for proper EZH2 (enhancer of zeste homolog 2)-mediated methyltransferase activity. It also associates with PRC1 (polycomb repressive complex 1) and helps in bringing PRC1 to H3K27me3 (trimethylated lysine residue at position 27 in the protein histone H3) loci. EED also increases PRC1-mediated H2A (histone 2A) ubiquitin E3 ligase activity. Mutations in the EED gene are associated with overgrowth, intellectual disability and dysmorphic features. Change in the activity of EED is linked with the pathogenesis of myeloid disorders.

物理的形状

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注された希釈前の酵素を-70°Cで保存してください。注記:酵素は凍結解凍の繰り返しに非常に弱いので、注意してください。