SRE0023

β-Glucuronidase from abalone

Purified, aqueous solution, β-glucuronidase 150,000-250,000 units/mL, β-glucuronidase ≥20,000,000 units/g protein

• Enzyme Commission number: 3.1.2.31 (BRENDA, IUBMB)
• MDL番号: MFCD00130629
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 品質水準: 200
• 形状: aqueous solution
• β-glucuronidase activity: ≥20,000,000 units/g protein; 150,000-250,000 units/mL
• secondary activity: ≤7,500 units/mL sulfatase
• 保管温度: 2-8°C

詳細

アプリケーション

Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.

生物化学的／生理学的作用

β-Glucuronidase (GUSB) is a lysosomal enzyme, that degrades glucuronate-containing glycosaminoglycans. β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations. Absence of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain.

単位の定義

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

物理的形状

Supplied as an aqueous solution containing L-proline, ammonium acetate and sodium azide

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable