SAB4200826

Anti- Cholera Toxin-Peroxidase antibody produced in rabbit

IgG fraction of antiserum

• 別名: Cholera enterotoxin subunit B (ctxB), Cholera enterotoxin, A chain (ctxA)
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: peroxidase conjugate
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 詳細: Research area: Microbiome
• 形状: lyophilized powder
• 分子量: subunit mol wt ~12 kDa (non-toxic CTxB ); subunit mol wt ~27 kDa (the toxic CTxA )
• 化学種の反応性: Vibrio cholerae
• 濃度: ~4 mg/mL
• テクニック: direct ELISA: 1:8,000-1:16,000 using 5 μg/ml Cholera Toxin for coating
• UniProtアクセッション番号: P01555 (ctxA); P01556 (ctxB)
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: Vibrio cholerae ... ctxA(57740120) , ctxB(57740119)

詳細

詳細

Cholera toxin (CTx) also known as choleragen, is an enterotoxin produced by the Gram-negative bacterium Vibrio cholerae that naturally habitat in fresh or saltwater environments. CTx is composed of two subunits, the toxic CTxA (~27 kDa) and non-toxic CTxB (~12 kDa) assembled with the stoichiometry AB5. The V. cholerae virulence factors CtxA and CtxB are located at the CTX phage genome integrated within the bacterial chromosome.

特異性

Anti-Cholera Toxin-peroxidase antibody specifically recognizes Cholera Toxin and has no cross reactivity with Staphylococcal Enterotoxin A (SEA), Staphylococcal Enterotoxin B (SEB) or Pseudomonas Exotoxin A

免疫原

purified toxin from Vibrio cholera

アプリケーション

Anti- Cholera Toxin-Peroxidase antibody produced in rabbit has been used in direct enzyme linked immunosorbent assay.

生物化学的／生理学的作用

The A1 fragment of the cholera toxin (CTx)A subunit is translocated through the membrane of the host cell, where it catalyses the ADP-ribosylation of the α-subunit of Gs (Gsa), a regulatory component of the adenylate cyclase complex. The resulting increased level of cyclic AMP promotes a wide variety of actions, including the secretion of chloride ions in the case of intestinal epithelial cells. Most of the V. cholerae species do not cause any disease in human, but few including serotypes O1 and O139 can cause cholera pandemics. Antibodies specific for cholera toxin may be used in studies of structural and functional aspects of toxin membrane interactions and for the detection of CTxB, when used, for example, as an adjuvant when injected mucosally together with the desired antigen.

物理的形状

Supplied as a lyophilized powder

保管および安定性

Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable