Calcineurin (a-subunit) (CnA), also known as Serine/threonine-protein phosphatase 2B catalytic subunit alpha (PP3CA), Calmodulin-dependent calcineurin A subunit alpha (CALN/CALNA) or CAM-PRP catalytic subunit, is a major soluble calmodulin-binding protein in the brain. Calcineurin is a heterodimer metalloenzyme, Ca2+/Calmodulin dependent serine/threonine protein phosphatase 3 (PPP3), with a relatively narrow substrate specificity4-6,8 It is composed of two subunits, a catalytic calmodulin-binding a subunit (61 kDa, calcineurin A [CnA]) and regulatory calcium-binding ß subunit (18 kDa, calcineurin B [CnB]).6,7 Both subunits are highly conserved from yeast to mammals.8
特異性
Monoclonal Anti-Calcineurin specifically recognizes an epitope located on the a-subunit of calcineurin from human, mouse, monkey, porcine1, rat2 and bovine13 origin. It does not cross-react with calcineurin ß-subunit.
免疫原
Purified bovine brain calcineurin.
アプリケーション
The antibody may be used in various immunochemical techniques including Immunoblot1,2 (~61 kDa), ELISA and Immunohistochemistry1,3.
物理的形状
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
保管および安定性
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Pathobiology : journal of immunopathology, molecular and cellular biology, 83(6), 308-315 (2016-06-21)
Increased expression of calcineurin in colorectal cancer (CRC) has been reported. Although the oncogenic function has been suggested, the clinical relevance is still unclear. We herein studied calcineurin expression as a prognostic biomarker in patients receiving curative surgery for stages
