S1816

SYBR^®Green JumpStart^™Taq ReadyMix^™ 定量PCR用, キャピラリー組成

SYBR^® Green qPCR reagent for Roche LightCycler^® capillary systems

• UNSPSCコード: 41106300
• NACRES: NA.55

特性

• フォーム: liquid
• 使用法: sufficient for 100 reactions; sufficient for 400 reactions
• 特徴: dNTPs included; hotstart
• 濃度: 1 units/reaction (20 μL reaction volume)
• テクニック: qPCR: suitable
• 色: colorless
• 入力: purified DNA
• 適合性: for use with Roche LightCycler 480
• 検出方法: SYBR^® Green
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

SYBR^® Green JumpStart^™ Taq ReadyMix^™, Capillary formulation combines the advantages of a hot start enzyme, JumpStart Taq, in a 2× concentrate ReadyMix specifically designed for use with capillary instruments, such as the Roche LightCycler^® real-time thermal cycler. SYBR Green JumpStart Taq ReadyMix is an optimized formulation containing SYBR Green I dye, JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer and stabilizers.

SYBR Green Taq ReadyMix is recommended for single product real-time amplification experiments and may also be used for evaluation of primer sequences prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.

アプリケーション

SYBR^® Green JumpStart^™ Taq ReadyMix^™ for Quantitative PCR, Capillary Formulation has been used in a real-time quantitative polymerase chain reaction (RT-qPCR) and reverse transcription PCR (RT-PCR).

特徴および利点

• Convenient 2× concentrate ReadyMix specifically designed for use with capillary instruments such as the Roche LightCycler^® and is ideal for high throughput applications
• Increased specificity & target yield - JumpStart^™ Taq polymerase prevents non-specific product resulting in more accurate C_T values and improved quantitation
• This master mix allows consistency from one reaction to the next
• Designed to reduce the preparation time and minimize contamination from multiple pipetting steps
• The double-strand DNA-specific SYBR^® Green I fluorescent dye is inexpensive, easy to use, and sensitive and is ideal for quantifying any DNA sequence

包装

A tube of 25 mM MgCl₂ is provided for easy optimization of the QPCR reaction.

Default reaction volume is 20 μL

100RXN is packaged as 1 X 1 mL
400RXN is packaged as 1 X 4 mL

原理

SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.

The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate C_T values.

To prepare a reaction, 10 μL of ReadyMix is added to primers, template and water for a final reaction volume of 20 μL.

単位の定義

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

法的情報

本製品を使用する場合、米国特許番号 5,994,056、6,171,785と、対応する米国外での特許請求のうち、1つ以上が適用されます。本製品の購入について、購入者自身の内部研究のために購入した量を使用する場合に限り、前述の特許請求に基づく訴訟から免責されますが、これは限定的なものであり、譲渡することはできません。他の特許請求(米国特許番号6,814,934に含まれる装置やシステムなど)によって保護されている権利や、あらゆる種類の商業サービス(購入者の研究結果を報酬やその他の商業的事由のために無制限に報告することを含む)を行う権利が、暗示や禁反言によって明示的に譲渡されるわけではありません。本製品は研究専用です。Rocheの特許に基づき診断に利用する場合は、別途Rocheからライセンスを受ける必要があります。ライセンス購入に関する詳細な情報については、ライセンス管理者(Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA)にお問い合わせ下さい。

JumpStart is a trademark of Sigma-Aldrich Co. LLC

LightCycler is a registered trademark of Roche

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable