PLA0071

Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

• 別名: 32kDa, REPA2, RF-A protein 2, RP-A p32, RP-A p34, RPA32, replication factor A protein 2, replication protein A 34 kDa subunit, replication protein A2, replication protein A2 (32kD)
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• 交差性: human, mouse
• テクニック: immunocytochemistry: 1:500-1:5,000; immunohistochemistry: 1:500-1:2,000; immunoprecipitation (IP): 2-10 μg/mg; western blot: 1:2,000-1:10,000
• アクセッション番号: NP_002937.1
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: phosphorylation (pSer4/pSer8)
• 遺伝子情報: human ... RPA2(6118)

詳細

詳細

Replication protein A2 (RPA32) is encoded by the gene mapped to human chromosome 1p35.3. It is a subunit of the heterotrimeric replication protein A (RPA) complex.

免疫原

Immunogen was a dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.1 (GeneID 6118).

生物化学的／生理学的作用

Replication Protein A (RPA) binds single-stranded DNA and plays a vital role in DNA replication, DNA repair, and checkpoint signaling. Phosphorylation of replication protein A2 (RPA32) by ataxia telangiectasia and Rad3-related protein kinase (ATR) and DNA-dependent protein kinase (DNA-PK) causes checkpoint kinase 1 (Chk1) activation and replication arrest. DNA-PK and ATR signaling via RPA32 (S4/S8) enhances genome stability and cell survival following replication stress.

物理的形状

トリスクエン酸／リン酸塩緩衝液、pH 7～8、0.09％ アジ化ナトリウム含有

その他情報

Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable