The long term stability of this product in solution is not been investigated. However, aqueous solutions are expected to be stable for at least 6 months when stored in working aliquots at -20°C. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/143/182/p9582pis.pdf
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アプリケーション
TLR3 は二重鎖 RNA を認識し、ウイルスに対する免疫応答の主なエフェクターです。 ポリイノシン-ポリシチジル酸（ポリ（I）・ポリ（C））は、TLR3 レベルでの細胞シグナルを研究するためのモデル RNA として使用される二重鎖ホモポリマーです。 ポリ（IC）は、TRIF 依存的なトール様受容体 3（TLR3）のリガンドです。
生物化学的／生理学的作用
ポリ(I:C)のNIT-1細胞へのトランスフェクションは、細胞内のdsRNAにより誘発されるβ細胞アポトーシスモデルとして用いられています。トランスフェクション後18時間で、NF-κB、p50/p65の核移行、カスパーゼ3および8の切断、カスパーゼ12、Fas、IL-15、TNF受容体関連リガンド(TRAIL)の転写誘導の増加により、細胞の45%がアポトーシスを起こしました。ポリ(I:C)は、安定した成熟樹状細胞(DC)の最も適切な生成因子の1つであることが示唆されています。注入後、この成熟DCは、CD40連結後の生理活性物質IL-12の分泌能により、in vivoにおいて効果的な免疫応答を引き起こすと考えられます。ポリ(I:C)は、ペプチドベースのワクチンに対する特定の抗腫瘍免疫応答強化のため、強力なアジュバントとして用いられました。
包装
容量は、ポリヌクレオチド含量に基づいています。
その他情報
二本鎖ホモポリマ-
調製ノート
本剤には二本鎖構造を維持するためのイオン強度が必要です。約10 mg/mLの水で再構成し、ポリヌクレオチドの生理的PBS溶液を調製します。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
P9582-BULK:
P9582-50MG-PW:
P9582-50MG:
P9582-5MG-PW:
P9582-5MG:
P9582-VAR:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
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Halime Kalkavan et al.
Nature communications, 8, 14447-14447 (2017-03-02)
Immune-mediated effector molecules can limit cancer growth, but lack of sustained immune activation in the tumour microenvironment restricts antitumour immunity. New therapeutic approaches that induce a strong and prolonged immune activation would represent a major immunotherapeutic advance. Here we show
William R Crum et al.
Brain, behavior, and immunity, 63, 50-59 (2016-12-13)
Genetic and environmental risk factors for psychiatric disorders are suggested to disrupt the trajectory of brain maturation during adolescence, leading to the development of psychopathology in adulthood. Rodent models are powerful tools to dissect the specific effects of such risk
Tasnim Rahman et al.
Scientific reports, 10(1), 1982-1982 (2020-02-08)
People with schizophrenia exhibit deficits in inhibitory neurons and cognition. The timing of maternal immune activation (MIA) may present distinct schizophrenia-like phenotypes in progeny. We investigated whether early gestation [gestational day (GD) 10] or late gestation (GD19) MIA, via viral
Flavia S Mueller et al.
Brain, behavior, and immunity, 80, 406-418 (2019-04-14)
Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses. Evidence from investigations in
Wei-Li Wu et al.
Brain, behavior, and immunity, 62, 11-23 (2016-11-14)
Epidemiological studies show that maternal immune activation (MIA) during pregnancy is a risk factor for autism. However, mechanisms for how MIA affects brain development and behaviors in offspring remain poorly described. To determine whether placental interleukin-6 (IL-6) signaling is required
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What is the stability of this product in solution? For example, how long would a 5 mg/mL solution last when stored at -20 celcius?
1 回答-
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What is the size (bp) of this product?
1 回答-
This product has a estimated size range of 272 - 4242 bases with a resulting molecular weight range of 90,000 - 1,400,000 daltons.
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Does the product P9582-5MG contain 5mg of polyI:C or 500ug (10%) of poly I:C and 4.9mg buffer salts?
1 回答-
This product contains 10% Poly (I:C) with sodium chloride and sodium phosphate buffer salts. Please see the link below to a sample Certificate of Analysis for further details regarding concentration of the Poly (I:C).
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P9582-50MG0000213739.pdf役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How stable is Product P9582, Polyinosinic-polycytidylic acid potassium salt, in solution?
1 回答-
RNA and synthetic RNA analogues will be very stable in aqueous solution as long as the solutions are not contaminated with RNase.
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What is the molecular weight of Product P9582, Polyinosinic-polycytidylic acid potassium salt?
1 回答-
The molecular weight may vary from lot to lot with a range from 90,000 to 1,400,000 with an average of 200,000 to 500,000.
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Why is the total mass of Product P9582, Polyinosinic-polycytidylic acid potassium salt, in my bottle much greater than the designated package size?
1 回答-
The package size is based on the polynucleotide content. However, since the polynucleotide is supplied with sodium chloride and sodium phosphate buffer salts, its mass represented by the package size is approximately 10% of the total mass present in the bottle.
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Should Polyinosinic-polycytidylic acid potassium salt Product P9582 or P1038 be used for cell activation?
1 回答-
For cell activation, synthetic double-stranded RNA is widely used as ligand of Toll-like receptor 3 (TLR3) in place of what is supposed to be the natural ligand (viral dsRNA). The double stranded RNA homopolymer, i.e. Poly(I):Poly(C), Prod. No. P9582 can be used for this application. P1038 is a single-stranded RNA and will not activate TLR3.
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What is the difference between Polyinosinic-polycytidylic acid potassium salt Products P9582, P1038, P1530 and P0913?
1 回答-
Prod No. P9582 as well as Prod. Nos. P1530 and P0913 are double-stranded with one strand of Poly(I) and one strand of Poly(C). Prod. No. P1038 is a single-stranded RNA analog with C and I randomly making up the polynucelotide strand. Double-stranded Prod. No. P9582 is a potassium salt while double-stranded Prod. Nos. P0913 and P1038 are sodium salts. Prod. No. P0913 is gamma-irradiated. None of the other products have been gamma-irradiated. Unlike the other products, only approximately 10% of the total mass of Prod. No. P9582 is actually polynucleotide. The remaining 90% is buffer salts as sodium chloride and sodium phosphate.
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