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出荷可能日2025年4月02日詳細
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buffered aqueous glycerol solution
比活性
5-25 units/mg protein (BCA)
濃度
≥0.5 mg/mL
その他の活性
lactic dehydrogenase ≤0.5%
保管温度
−20°C
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アプリケーション
Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.
The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.[1]
生物化学的／生理学的作用
Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA[2].
単位の定義
1ユニットは、pH 7.8、30°C、1分間で、1.0 μmolのピルビン酸とCO2をオキサロ酢酸に変換する酵素量です。
物理的形状
0.05 M Tris-HCl(pH 7.4), 2 mM酢酸マグネシウム, 1 mM EDTAを含有する50%グリセロ-ルに溶解された溶液
調製ノート
アフィニティ-精製
保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
P7173-100UN:
P7173-25UN:
P7173-10UN:
P7173-BULK:
P7173-VAR:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
Philip Lee et al.
Hepatology (Baltimore, Md.), 57(2), 515-524 (2012-08-23)
The pathogenesis of type 2 diabetes is characterized by impaired insulin action and increased hepatic glucose production (HGP). Despite the importance of hepatic metabolic aberrations in diabetes development, there is currently no molecular probe that allows measurement of hepatic gluconeogenic
Eunsook S Jin et al.
Metabolism: clinical and experimental, 62(1), 152-162 (2012-09-18)
A three-day high-fat diet induces hepatic steatosis and hepatic insulin resistance in rats without altering fasting plasma glucose concentration or the rate of glucose production. However, as the nutrient profile available to the liver is substantially altered by a high-fat
Scott B Crown et al.
BMC systems biology, 6, 43-43 (2012-05-18)
¹³C-Metabolic flux analysis (¹³C-MFA) is a standard technique to probe cellular metabolism and elucidate in vivo metabolic fluxes. 13C-Tracer selection is an important step in conducting ¹³C-MFA, however, current methods are restricted to trial-and-error approaches, which commonly focus on an
H M White et al.
Journal of dairy science, 95(3), 1249-1256 (2012-03-01)
Expression of mRNA for pyruvate carboxylase (PC) is elevated at calving and during other physiological states when plasma NEFA concentrations are increased. The objective of this study was to determine the direct effects of fatty acids on expression of PC
Abdussalam Adina-Zada et al.
Biochemistry, 51(41), 8208-8217 (2012-09-19)
Mutation of Arg427 and Arg472 in Rhizobium etli pyruvate carboxylase to serine or lysine greatly increased the activation constant (K(a)) of acetyl CoA, with the increase being greater for the Arg472 mutants. These results indicate that while both these residues
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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