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タイプ
Type XXI
形状
powder
比活性
≥15 units/mg solid
分子量
~50 kDa
保管温度
−20°C
関連するカテゴリー
アプリケーション
Pronase E can be used to degrade antheraea pernyi silk fibroin films.
A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
Completely inactivated by heating above 80 °C for 15-20 minutes.
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein. Proteases from Streptomyces griseus and Streptomyces omiyaensis have been used in a study to identify residue 71 as a crucial residue for differences in topological specificities. Proteases have also been used in a study to investigate production of protease as a by-product of streptomycin production.
生物化学的／生理学的作用
アルカリプロテアーゼは、pH 11.0で、プロテアーゼの通常のアッセイ条件であるpH 7.5、37°Cの場合と比べて約2倍の活性があります。比較すると、P5147タイプXIVプロテアーゼは、pH 11.0、30°Cで約25%の活性しかありません。
物性
Isoelectric point : 8.7
Inhibitors : Diisopropyl fluorophosphate, EDTA
Optimum pH : >=12
Optimum temperature : 60oC
pH Stability : pH 5.0 - 11.5 (25oC,24hr)
Thermal stability : below 50oC (pH 8.3, 15min)
Inhibitors : Diisopropyl fluorophosphate, EDTA
Optimum pH : >=12
Optimum temperature : 60oC
pH Stability : pH 5.0 - 11.5 (25oC,24hr)
Thermal stability : below 50oC (pH 8.3, 15min)
単位の定義
1ユニットは、pH 11.0、30°C、1分間に、カゼインを加水分解して、1.0 μmol(181 μgのチロシンに相当するペプチドを生成する酵素量です。
物理的形状
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
調製ノート
Collected from culture broth of S.Sp
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
ターゲットの組織
Respiratory system
保管分類コード
11 - Combustible Solids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
P0652-100UN:
P0652-500UN:
P0652-VAR:
P0652-1KU:
P0652-BULK:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Yoshiko Uesugi et al.
Biochimica et biophysica acta, 1814(10), 1295-1304 (2011-07-20)
We recently identified residue 71 of two homologous serine proteases from Streptomyces omiyaensis (SOT) and Streptomyces griseus (SGT) as a crucial residue for differences in their topological specificities, i.e. recognition of a distinct three-dimensional structure. To study the role of
Paola Taddei et al.
Biomacromolecules, 7(1), 259-267 (2006-01-10)
In this study, Antheraea pernyi silk fibroin (Ap-SF) films were incubated with Protease Type XXI from Streptomyces griseus, at 37 degrees C, to investigate the degradation behavior in an in vitro model system. The enzyme-resistant fractions of Ap-SF films and
A Proteolytic Enzyme of Streptomyces griseus: I. Purification of a Protease of Streptomyces griseus
Nomoto, M. and Y. Narahashi
Biochemistry, 46, 653-667 (1959)
The utility of nonspecific proteases in the characterization of glycoproteins by high-resolution time-of-flight mass spectrometry.
Juhasz, P. and Martin, S.A.
International Journal of Mass Spectrometry and Ion Processes, 169-170, 217-230 (1997)
W W Epstein et al.
Proceedings of the National Academy of Sciences of the United States of America, 87(19), 7352-7354 (1990-10-01)
Prenylated proteins, labeled in the isoprenoid residue by growing CHO cells in medium containing [5-3H]mevalonate, were degraded by three different proteolytic procedures, enzymatic or alkaline hydrolysis as well as hydrazinolysis. The products thus obtained were analyzed by HPLC with chemically
プロトコル
This procedure is for informational purposes including assay Procedure, definition, and calculations for Protease
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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