M8065

O⁶-メチルグアニン-DNAメチルトランスフェラーゼ ヒト

≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ≥10,000 units/mg protein

• 別名: AGT, MGMT
• MDL番号: MFCD03792500
• UNSPSCコード: 12352200

特性

• リコンビナント: expressed in E. coli
• アッセイ: ≥90% (SDS-PAGE)
• 形状: buffered aqueous glycerol solution
• 比活性: ≥10,000 units/mg protein
• 分子量: ~21 kDa; apparent mol wt ~25 kDa by SDS-PAGE
• UniProtアクセッション番号: P16455
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... MGMT(4255)

詳細

生物化学的／生理学的作用

O⁶-Methylguanine-DNA methyl transferase (MGMT), containing 207 amino acids, is a ubiquitous DNA repair enzyme that removes O⁶-alkyl-guanine, primarily O⁶-methylguanine, lesions from damaged DNA.

O⁶-Methylguanine-DNA methyl transferase (MGMT), containing 207 amino acids, is a ubiquitous DNA repair enzyme that removes O⁶-alkyl-guanine, primarily O⁶-methylguanine, lesions from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation. Its action is based on the transfer of the alkyl group from the DNA to a unique acceptor cysteine residue in the protein, forming a stable thioether linkage. The cysteine sulfhydryl moiety is not regenerated and therefore, MGMT is frequently classified as a suicide enzyme. Since the reaction irreversibly inactivates the enzyme, the repair capacity for O⁶-methylguanine is dependent on the number of MGMT molecules in the cell. There is a correlation between the occurrence of cancer in various tissues and the lack of the MGMT enzyme. High levels of MGMT result in reduced tumor events and resistance of tumors to alkylating agents.

単位の定義

1ユニットは、37°Cで10分間に、⁶-メチルグアニン含有の二本鎖DNAオリゴヌクレオチド基質0.5 nmolのメチル基の50%を除去するタンパク質量です。

物理的形状

Solution in 50% glycerol containing 20 mM Tris, pH 7.5, 1 mM EDTA, 200 mM NaCl, and 1 mM DTT.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)