M7318

Anti-MBD2a,b (RA-18) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 別名: Anti-DMTase, Anti-NY-CO-41
• MDL番号: MFCD06798469
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 交差性: mouse, human
• テクニック: indirect immunofluorescence: 6-8 μg/mL using NIH3T3 cells; microarray: suitable; western blot: 0.5-1.0 μg/mL using nuclear extracts of HeLa cells
• UniProtアクセッション番号: Q9UBB5
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MBD2(8932) ; mouse ... Mbd2(17191)

詳細

詳細

Anti-MBD2a,b (RA-18) is developed in rabbit using as immunogen a synthetic peptide corresponding human MBD2a conjugated to KLH. MBD2 consists of two isoforms, MBD2a and MBD2b, which are generated from a single gene; MBD2a is a 414 amino acids protein, whereas MBD2b lacks the 152 amino acids N-terminal extension. MBD2a is a component of the MeCP1 corepressor complex, which is a 400-800 kDa complex containing as components MBD2, Mi-2, MTA2, MBD3, and HDAC1/2.

The gene encoding methyl-CpG binding domain protein 2 (MBD2a) is localized on chromosome 18.

特異性

The antibody recognizes MBD2a (~45 kDa) and MBD2b (~29 kDa). In immunoblotting, an unidentified band at ~43 kDa may also be detected.

免疫原

synthetic peptide corresponding to amino acids 294-411 of human MBD2a conjugated to KLH via an N-terminal added lysine residue. This sequence is conserved in MBD2a and MBD2b of human origin, and is not found in MBD3.

アプリケーション

Anti-MBD2a,b (RA-18) antibody produced in rabbit has been used:

• for chromatin immunoprecipitation and western blotting
• immunofluorescence
• immunoblotting

生物化学的／生理学的作用

MBD2 binds to methylated DNA in vitro or in vivo, suggesting that MBD2 targets the MeCP1 complex to methylated DNA. MBD2a interacts with RNA Helicase A (RHA). MBD2 protein appear to protect against some cancers.

Methyl-CpG binding domain protein 2 (MBD2a) acts as a repressor during transcription.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable