M5909

IgMアイソタイプコントロール マウスミエローマ由来

clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin

• 別名: マウスIgMアイソタイプコントロール ミエローマ由来
• MDL番号: MFCD00164813
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• クローン: MOPC 104E, monoclonal
• 形状: buffered aqueous solution
• 濃度: 200 μg/mL
• テクニック: flow cytometry: suitable
• アイソタイプ: IgMλ
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory . This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice . IgM isotype control antibody is specific for anti-mouse whole serum and anti-mouse IgM. The product does not react with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3.

Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.

The purified IgM Isotype Control clone MOPC 104E preparation is non-reactive with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3. When evaluated in flow cytometry, the IgM Isotype Control did not stain human peripheral blood lymphocytes (PBLs). A FITC Goat anti-Mouse IgM (μ-chain specific), Affinity Isolated Antibody (Product No. F 9259) along with 1 μg of the product was incubated with human PBLs and then evaluated by flow cytometry.

アプリケーション

IgM Isotype Control clone MOPC 104E is recommended as a negative control in flow cytometry. Working Concentration: equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry. Equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry.

IgM Isotype Control from murine myeloma has been used:

• in chromatin immunoprecipitation (ChIP) assay
• LABScreen assays
• immunofluorescent staining

IgM isotype control antibodies are suitable for use in immunohistochemistry .

原理

標的細胞群に対する試薬の非特異的結合を測定し、マウスCD抗原に対するモノクロ-ナル抗体の染色の特異性を確認する必要があります。イムノアッセイに用いるモノクロ-ナル抗体に対する陰性対照として、濃度・アイソタイプの適合したマウス骨髄腫イムノグロブリンを用いる方法があります。
Sigmaのマウスアイソタイプコントロ-ルは、Sigmaの精製済み抗CD抗原モノクロ-ナル抗体(標識・未標識)の濃度と合っています。DUAL-TAGコントロ-ルは、各複合体をそれぞれ100μg含みます。

物理的形状

0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable