L6893

Lipopolysaccharides (rough strains) from Escherichia coli F583 (Rd mutant)

• 別名: LPS
• CAS番号: 93572-42-0
• UNSPSC Code: 12352201
• EC Number: 297-473-0
• NACRES: NA.25
• MDL number: MFCD00164401

特性

• biological source: Escherichia coli (F583 Rd mutant)
• Quality Segment: 200
• form: lyophilized powder
• impurities: <3% Protein (Lowry)
• color: white to yellow cast
• solubility: water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow
• shipped in: ambient
• storage temp.: 2-8°C

詳細

General description

This product is phenol:chloroform:petroleum ether extracted from E. coli F 583 (Rd mutant). The source strain is from a private collection.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

安全性情報

• ピクトグラム: GHS06
• 注意喚起語: Danger
• ハザードコード: H300
• 注意事項: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 2 Oral
• 保管分類: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• 一体何が起きているの？: WGK 3
• 引火点 (F): Not applicable
• 引火点（°C）: Not applicable
• PPE（個人用保護具）: Eyeshields, Gloves, type N95 (US)