GE29-0486-09

GSTrap^™ 4B

Cytiva 29-0486-09, pack of 1 mL

• UNSPSCコード: 23151817
• NACRES: NA.56

特性

• ligand: glutathione
• 包装: pack of 1 mL
• メーカー／製品名: Cytiva 29-0486-09
• 利用可能性: not available in North America
• パラメーター: 42 psi (H₂O at 25°C)
• ベッドサイズ: 7 mm × 25 mm
• ベッド容積: 1 mL
• カラム内径: 7 mm
• Matrix: 4% agarose
• 粒径: 45-165 μm
• 平均直径: 90 μm
• cleaning: 4-13
• 動作範囲: 4-13
• キャパシティ: >25 mg binding capacity (horse liver GST/ml medium)(H2O at 25°C)

詳細

詳細

GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

アプリケーション

Tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC System.

特徴および利点

• High binding capacity.
• Mild elution conditions preserving protein antigenicity and function.
• Easy one-step purifications of Glutathione S-Transferase (GST) tagged proteins resulting in high purity.
• GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as AKTA design.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

GSTrap is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids