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ligand
recombinant protein G lacking albumin-binding region
特徴
wetted part: no
包装
pack of 16 columns
メーカー/製品名
Cytiva 28-9031-34
保管条件
(20% Ehtanol)
(20% Ethanol)
Matrix
highly cross-linked 6% agarose
平均部品サイズ
34 μm
cleaning
2-9
動作範囲
3-9
キャパシティ
>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
>1 mg binding capacity(human IgG/column)
保管温度
2-8°C
関連するカテゴリー
詳細
アプリケーション
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
特徴および利点
- For simple, small-scale purification of antibodies or enrichment of specific Proteins in prepacked spin columns that are ready to use.
- Classic and cross-link protocols provide flexibility.
- Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
- Fast binding kinetics and high capacity provides high yield.
- Prepacked with Protein G Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- Easy scale-up with HiTrap® Protein G HP prepacked columns.
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
プロトコル
This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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