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GE27-5325-01

Microspin™ G-25 Columns

Cytiva 27-5325-01

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About This Item

UNSPSCコード:
41105501
NACRES:
NA.52
現在、価格および在庫状況を閲覧できません。

物質

resin (Sephadex G-25 DNA grade F)

無菌性

non-sterile

メーカー／製品名

Cytiva 27-5325-01

包装

pkg of 50 columns

有効容積

10 —100 μL

粒径

20-80 μm (dry)

ポアサイズ

5000  Da MWCO

動作範囲

2-13

関連するカテゴリー

詳細

MicroSpin™ G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.

MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.

特徴および利点

  • For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin-column chromatography.
  • Excellent for rapid purification of newly synthesized oligonucleotides >10-mers in 100 to 150 μl of deprotection solution using spin-column chromatography.
  • Convenient: Prepacked with Sephadex® G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
  • Ready to use: Requires less than 4 min from sample application to collection of purified product.
  • Tested in nickase, single and double-stranded exonuclease and RNase assays.
  • Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 μl.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 20 to 25 °C. (Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

MicroSpin is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

保管分類コード

12 - Non Combustible Liquids

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資料

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

プロトコル

DNA Cleanup using illustra™ GFX Kits

DNA cleanup using illustra™ GFX kits from Cytiva.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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