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ligand
octylamine
包装
pkg of 1 column
メーカー／製品名
Cytiva 17-5481-15
パラメーター
0.8-3.9 ml/min flow rate
8 bar
ベッドサイズ
77 mm × 100 mm
ベッド容積
4.7 mL
カラム内径
7.7 mm
Matrix
highly cross-linked agarose
平均部品サイズ
75 (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
3-13(pH interval where the medium can be subjected to, for cleaning- or sanitization-in-place.)
動作範囲
2-14(pH interval where the medium can be operated without significant change in function.)
キャパシティ
~13 mg binding capacity (ovalbumin)
適合性
suitable for bioprocess medium
詳細
Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules. The novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality, size exclusion, and binding chromatography in one chromatography resin.
Capto Core 700 is composed of a ligand-activated core and inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 700 000 [700 kDa]) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands. The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations. Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.
Capto Core 700 is composed of a ligand-activated core and inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 700 000 [700 kDa]) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands. The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations. Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.
Dynamic binding capacities were measured at 10% breakthrough with a residence time of 3 min (1.6 ml/min = 200 cm/h) in HiScreen columns. Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.
特徴および利点
- For intermediate purification and polishing of viruses and other large biomolecules (Mr > 700 000) in flowthrough mode.
- Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough.
- Significantly improved productivity and higher flow rates compared with gel filtration methods.
- Straightforward optimization due to flowthrough chromatography and robust performance.
- Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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