おすすめの製品
包装
pack of 25 mL
メーカー/製品名
Cytiva 17-5268-01
Matrix
highly cross-linked 6% agarose
平均部品サイズ
34 μm
cleaning in place
2-14(Ni2+-stripped medium.)
動作範囲
3-12(Ni²⁺-stripped medium.)
キャパシティ
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
関連するカテゴリー
詳細
Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
特徴および利点
- High-performance purification of histidine-tagged proteins
- Negligible leakage of Ni2+ ions.
- High binding capacity, at least 40 mg/mL medium
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- 34 μm bead size for improved resolution
保管および安定性
アナリシスノート
法的情報
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
最新バージョンのいずれかを選択してください:
資料
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
プロトコル
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
This page shows how to purify protein A-tagged proteins using Cytiva products.
How to isolate proteins from inclusion bodies using Cytiva products.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら(テクニカルサービス)