おすすめの製品
ligand
recombinant protein A (E. coli)
包装
pack of 1 × 5 mL
メーカー/製品名
Cytiva 17-5080-01
保管条件
(20% Ehtanol)
(20% Ethanol)
パラメーター
42 psi
ベッドサイズ
16 mm × 25 mm
ベッド容積
5 mL
カラム内径
16 mm
マトリックス
4% cross-linked agarose
粒径
60-165 μm
平均直径
90 μm (d50v)
cleaning
2-11
working range
3-10
キャパシティ
~50 mg binding capacity (human lgG/ml medium)
適合性
suitable for bioprocess medium
保管温度
2-8°C
詳細
The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
特徴および利点
- Quick, convenient preparative purification of monoclonal antibodies.
- Well-established protein A-based medium used in many approved MAb processes.
- Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.
保管および安定性
アナリシスノート
その他情報
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
プロトコル
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
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