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Merck

GE17-5080-01

HiTrap® rprotein A Fast Flow

Cytiva 17-5080-01, pack of 1 × 5 mL

別名:

HiTrap rProtein, Protein A Fast Flow Column, rProtein A Fast Flow

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

包装

pack of 1 × 5 mL

メーカー/製品名

Cytiva 17-5080-01

保管条件

(20% Ehtanol)
(20% Ethanol)

パラメーター

42 psi

ベッドサイズ

16 mm × 25 mm

ベッド容積

5 mL

カラム内径

16 mm

マトリックス

4% cross-linked agarose

粒径

60-165 μm

平均直径

90 μm (d50v)

cleaning

2-11

working range

3-10

キャパシティ

~50 mg binding capacity (human lgG/ml medium)

適合性

suitable for bioprocess medium

保管温度

2-8°C

詳細

Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.
HiTrap® rProtein A FF columns are prepacked with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特徴および利点

  • Quick, convenient preparative purification of monoclonal antibodies.
  • Well-established protein A-based medium used in many approved MAb processes.
  • Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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資料

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

プロトコル

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

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