GE17-5079-02

HiTrap^® rProtein A Fast Flow

Cytiva 17-5079-02, pack of 2 × 1 mL

• UNSPSCコード: 23151816
• NACRES: NA.56

特性

• ligand: recombinant protein A (E. coli)
• 包装: pack of 2 × 1 mL
• メーカー／製品名: Cytiva 17-5079-02
• 保管条件: (20% Ehtanol); (20% Ethanol)
• パラメーター: <4 mL/min flow rate; 42 psi
• ベッドサイズ: 7 mm × 25 mm
• ベッド容積: 1 mL
• カラム内径: 7 mm
• マトリックス: 4% cross-linked agarose
• 粒径: 60-165 μm
• 平均直径: 90 μm (d50v)
• cleaning: 2-11
• working range: 3-10
• キャパシティ: ~50 mg binding capacity (human lgG/ml medium)
• 適合性: suitable for bioprocess medium
• 保管温度: 2-8°C

詳細

詳細

Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.

HiTrap^® rProtein A FF columns are prepacked with rProtein A Sepharose^™ Fast FLow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

アプリケーション

The recombinant Protein A ligand (produced in E. coli) is coupled to Sepharose^™ Fast FLow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

特徴および利点

• Quick, convenient preparative purification of monoclonal antibodies.
• Well-established Protein A-based medium used in many approved MAb processes.
• Recombinant Protein A exhibits similar Fc region specificity to that of native Protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA^® design.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids