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包装
pkg of 10 ea
メーカー/製品名
Cytiva 11-0033-99
ベッド容積
1 mL
カラム内径
15 mm
Matrix
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14(Ni2+-stripped medium)
動作範囲
3-12(Ni2+-stripped medium.)
キャパシティ
~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.)
~40 mg binding capacity(histidine-tagged protein)
適合性
suitable for bioprocess medium
関連するカテゴリー
詳細
The ready to use column is prepacked with Ni Sepharose™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.
His GraviTrap™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.
特徴および利点
- For fast and simple purification of histidine-tagged* proteins.
- Convenient, prepacked gravity-flow column.
- Allows direct purification of both clarified and unclarified cell lysates.
- High binding capacity; approximately 40 mg of pure protein per column.
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives.
保管および安定性
アナリシスノート
法的情報
関連製品
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
プロトコル
This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.
How to isolate proteins from inclusion bodies using Cytiva products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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