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Merck

GE11-0033-99

His GraviTrap

Cytiva 11-0033-99

別名:

His GraviTrap Column

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About This Item

UNSPSCコード:
12161501
NACRES:
NA.32
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包装

pkg of 10 ea

メーカー/製品名

Cytiva 11-0033-99

ベッド容積

1 mL

カラム内径

15 mm

Matrix

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14(Ni2+-stripped medium)

動作範囲

3-12(Ni2+-stripped medium.)

キャパシティ

~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.)
~40 mg binding capacity(histidine-tagged protein)

適合性

suitable for bioprocess medium

関連するカテゴリー

詳細

His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.

The ready to use column is prepacked with Ni Sepharose 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.

His GraviTrap columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.

特徴および利点

  • For fast and simple purification of histidine-tagged* proteins.
  • Convenient, prepacked gravity-flow column.
  • Allows direct purification of both clarified and unclarified cell lysates.
  • High binding capacity; approximately 40 mg of pure protein per column.
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

関連製品

製品番号
詳細
価格

ピクトグラム

Environment

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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試験成績書(COA)

Lot/Batch Number

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資料

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

プロトコル

This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.

How to isolate proteins from inclusion bodies using Cytiva products.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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