G6160

モノクロナール抗β-COP マウス宿主抗体

clone maD, ascites fluid

• 別名: Anti-BARMACS, Anti-COPB
• MDL番号: MFCD00164606
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: maD, monoclonal
• 含みます: 15 mM sodium azide
• 交差性: human, rat, hamster, monkey
• テクニック: indirect immunofluorescence: 1:80 using cultured Chinese hamster ovary (CHO) cells; microarray: suitable; western blot: 1:1,000 using a preparation of stacked Golgi membranes from rat liver
• アイソタイプ: IgG1
• UniProtアクセッション番号: P53618
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... COPB1(1315) ; rat ... Copb1(114023)

詳細

詳細

The coatomer (approx. 550kDa) consists of proteins designated α-, β-, γ-, and δ-COP, together with substoichiometric amounts of several other proteins.

特異性

抗体はβ-COPタンパク質(110 kDa)のエピト-プを認識し、免疫細胞化学ではゴルジ複合体の周辺を染色します。

免疫原

合成β-COPペプチドD1(アミノ酸701-715)のKLH結合体。

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-β-COP antibody produced in mouse is suitable for use as a primary antibody in immunoblot:

• analysis at a working dilution of 1:1000 using subcellular proteins from rat PC12 (pheochromocytoma) cells
• analysis of gradient fractions of cerebral microvessels to confirm the separation of plasma membrane lipid raft domains from intracellular membranous components
• detection of the Golgi marker protein β-COP in exosome-enriched extracellular microvesicles (eMV) preparations from untreated HeLa cells

It is suitable for immunostaining of β-coatomer, that is used as a intracellular, Golgi protein marker :

• to confirm that CD14 staining is localized to the cell surface of HAEC
• for examining the localization of Meltrin β in the Golgi apparatus and its vicinity in neurons prepared from developing dorsal root ganglia of mouse embryos
• in NB4 and NB4-LR1 cells to examine the colocalization of PKA regulatory subunits
• in CHO cells to examine colocalization of GFP-Rab24

It is suitable for use in cell-surface ELISA of human aortic endothelial cells (HAEC)

It is also suitable for western blot analysis at a working dilution of 1:1000 using a preparation of stacked Golgi membranes from rat liver, for indirect immunofluorescence at a working dilution of 1:80 using cultured Chinese hamster ovary (CHO) cells and for microarray.

生物化学的／生理学的作用

COPs (coatomer proteins) contain adaptin-like, complex (8) and are transiently attached to the vesicles involved in transport within the Golgi complex and possibly between the rough ER and Golgi complex. β-COP has a molar mass of 110kDa and its primary structure is homologous to the β-adaptin component of clathrin-coated vesicles.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable