G3512

グリセリンデヒドロゲナーゼ Cellulomonas sp.（セルロモナス）由来

lyophilized powder, ≥50 units/mg protein

• 別名: グリセリン:NAD⁺ 2-オキシドレダクターゼ
• Enzyme Commission number: 1.1.1.6 (BRENDA, IUBMB)
• EC Number: 232-824-3
• MDL番号: MFCD00131206
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: bacterial (Cellulomonas spp.)
• 形状: lyophilized powder
• 比活性: ≥50 units/mg protein
• 分子量: ~390 kDa
• 組成: Protein, ≥60% biuret
• 保管温度: −20°C

詳細

詳細

Glycerol Dehydrogenase belongs to iron-containing alcohol dehydrogenase family. It is made up of eight identical subunits which are about 42,000 Da each. The C-terminal has histidine and the N-terminal has serine amino acid.

アプリケーション

Glycerol Dehydrogenase (GDH) from Cellulomonas sp. has been used in the determination of glycerol by sequential injection analysis (SIA) method. It has also been used in the preparation of pyrroloquinoline quinone (PQQ) based biosensor for the determination of glycerol.

This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD⁺ was catalyzed by the enzyme glycerol dehydrogenase.

生物化学的／生理学的作用

Glycerol dehydrogenase catalyzes the conversion of glycerol to glycerone.

物性

Isoelectric point : 4.4 ± 0.1
Michaelis constants : 1.1 x 10¯²M (Glycerol), 8.9 x 10¯⁵M (NAD⁺⁾
Structure : 10 subunits (42,000) per mol of enzyme
Inhibitors : p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal
ions (Co++, Ni⁺⁺, Cu⁺⁺, Zn⁺⁺, Cd⁺⁺)
Optimum pH : 10.0 – 10.5
Optimum temperature : 50°C
pH Stability : pH 7.5 – 10.5 (25°C, 20hr)
Thermal stability : below 55°C (pH 7.5, 15min)
Substrate specificity : This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also oxidizes glycerol-α-monochlorohydrin, ethylene
glycol and 2,3-butanediol. The oxidative reaction is stimulated by K⁺, NH⁺4 and Rb⁺.

単位の定義

1ユニットは、pH 10.0、25°C、1分間で、1.0 μmolのグリセロールをジヒドロキシアセトンへと酸化する酵素量です。

物理的形状

凍結乾燥粉末(BSA含有)

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)