F6926

線維芽細胞増殖因子-8b マウス由来

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

• 別名: FGF-8b
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD01323276

特性

• biological source: mouse
• Quality Segment: 100
• recombinant: expressed in E. coli
• assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 15-60 ng/mL
• mol wt: calculated mol wt 23.6 kDa, predicted mol wt 22 kDa
• packaging: pkg of 25 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• UniProt accession no.: P37237
• storage temp.: −20°C
• Gene Information: mouse ... Fgf8(14179)

詳細

General description

Fibroblast growth factor 8 (FGF8) is a member of the FGF family, and was initially cloned from androgen-dependent mouse mammary carcinoma cell line. Alternative splicing of this gene produces four different isoforms mainly, FGF8a, FGF8b, FGF8e and FGF8f.[1]

Application

Fibroblast Growth Factor-8b from mouse has been used-

• for subgerminal cavity injection in fertilized quail, chicken, turtle and emu eggs[2]
• in the induction of neural differentiation in stem cells from Human Exfoliated Deciduous teeth (SHED)[3] and
• in experiments conducted on fertilized hens′ eggs, where FGF8 induced SOX3 gene.[4]

Biochem/physiol Actions

FGF-8bは、原腸形成後の胚に生じた外胚葉の分化、例えば顔、肢、中枢神経系の成長やパターン形成などに関与しています。FGF-8bは、FGFR-2、FGFR-3、FGFR-4のスプライス型「c」を活性化します。

FGF-8bは、顔、四肢や中枢神経系の成長やパターン形成など原腸形成後の胚の外胚葉分化に関与しています。FGF-8bはFGFR-2、FGFR-3、FGFR-4のC末端スプライス型を活性化します。

Fibroblast growth factor 8b (FGF8b) isoform of FGF8 has the most potent angiogenic and tumorigenic properties. In prostate cancer, it is responsible for angiogenesis, tumorigenesis and invasion. Exogenous expression of this gene in prostate epithelium leads to prostatic intraepithelial neoplasia (PIN). It also interacts with pattern recognition receptor long pentraxin-3 (PTX3), which is involved in local inflammation.[1]

Physical form

Lyophilized from a 0.2μm filtered solution in 20mM MOPS, 0.1M sodium sulfate, 0.02% Brij-35, pH 7 containing 50ug of BSA per 1μg as a carrier protein

Analysis Note

生物活性は、NR6R-3T3線維芽細胞の増殖刺激能で測定されています。

安全性情報

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319
• pcodes: P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• 保管分類: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)