D8312

REDTaq^® ゲノムDNAポリメラーゼ

with MgCl₂

• UNSPSC Code: 12352204
• NACRES: NA.55
• MDL number: MFCD01635818
• Concentration: 1 unit/μL

特性

• Quality Segment: 200
• form: liquid
• usage: sufficient for 1000 reactions, sufficient for 250 reactions, sufficient for 2500 reactions
• feature: Difficult Templates/Specialty Enzymes PCR, dNTPs included: no, hotstart: no
• concentration: 1 unit/μL
• technique(s): PCR: suitable, whole genome amplification: suitable
• color: red
• input: purified DNA
• application(s): agriculture
• shipped in: wet ice
• storage temp.: −20°C

詳細

General description

REDTaq^® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.

The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides (the building blocks of DNA). These enzymes are essential for DNA replication and usually work in pairs. It creates two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.

Application

REDTaq^® Genomic DNA Polymerase is used for routine PCR amplification of genomic DNA.

Features and Benefits

• Enhanced amplification on genomic and difficult DNA templates
• Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
• Quick recognition and confirmation of appropriate mixing
• No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
• The samples can be re-amplified as in nested PCR
• The red dye migrates faster than bromophenol blue
• Greater consistency across reactions due to proper mixing

Other Notes

1 unitは、74°C、30分間に、10 nmolの全dNTPを酸不溶性DNAに取り込む酵素量です。

Legal Information

本製品を購入しても、米国特許番号 5,804,375、5,994,056、6,171,785、6,214,979、5,538,848、5,723,591、5,876,930、6,030,787、6,258,569、対応する米国外の特許、5'ヌクレアーゼとdsDNA-Binding Dye Processに関連するその他の特許や特許出願に基づくライセンスは譲渡されません。さらに詳細な情報については、ライセンス管理者(Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA)にご連絡下さい。

REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類: 10 - Combustible liquids
• WGK: WGK 3
• 引火点 (F): Not applicable
• 引火点（°C）: Not applicable