This calf thymus DNA is a highly polymerized DNA containing both double-stranded and single-stranded forms, with double-stranded DNA being the predominant form. The length of the DNA is not determined. The base pair length of the starting material is at least 20 kilobases but the product is "activated" which means nicked for nick translation reaction and more fragile resulting in shorter lengths than the starting material.
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
50 mg | 在庫あり出荷可能from市川市大町368 | ¥14,500 |
100 mg | 在庫あり出荷可能from市川市大町368 | ¥17,400 |
500 mg | カートの在庫状況を確認する | ¥75,700 |
1 g | カートの在庫状況を確認する | ¥92,000 |
5 g | カートの在庫状況を確認する | ¥410,000 |
この商品について
General description
仔ウシ胸腺由来のDNAは、41.9モル%G-Cと58.1モル%A-Tです1。260 nmでのOD 1.0は、約50 μgの二本鎖DNAに相当します。
Application
Physical form
Preparation Note
Analysis Note
1 of 1
当該品目 | |||
|---|---|---|---|
| Quality Level 300 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| form fibers | form powder or crystals | form solid | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| color white | color - | color off-white | color - |
| type Type I | type - | type - | type - |
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保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
D1501-5G: + D1501-50MG: + D1501-500MG: + D1501-VAR: + D1501-1G: + D1501-100MG: + D1501-BULK: + D1501-EW:
jan
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What is the bp(length) of this ctDNA? and is it a mixture of ds and ss DNA ?
1 回答-
役に立ちましたか?
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What is the purity of the chemical available?
1 回答-
The purity of this material is determined by UV absorption at 260 nm. One milligram of DNA is equivalent to approximately 20 A260 units. This product is assigned a minimum A260 specification of 16. This information is lot specific and reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/d1501#product-documentation役に立ちましたか?
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This product is tested in 15 mM NaCl with 1.5 mM Sodium citrate, pH 7.0 using a 1 cm light path. One mg of DNA is equivalent to 20 A260 units. Is the sodium citrate monobasic, dibasic or tribasic? thank you
1 回答-
Sodium citrate tribasic dihydrate was used to test this product.
役に立ちましたか?
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How would you recommend soluting ctDNA for DNA determination? In Tris-EDTA (93302) or 15 mM NaCl and 1.5 mM Sodium Citrate? If we prepare the solution of 50 mg ctDNA + 50 ml Tris-EDTA and dilute it 1/20, would OD260 of 1.0 correspond to 50 ug/l? Thank you
1 回答-
This product is tested in 15 mM NaCl with 1.5 mM Sodium citrate, pH 7.0 using a 1 cm light path. One mg of DNA is equivalent to 20 A260 units. Using other buffers would not be recommended as the data could not be comparable to that reported in the product Certificate of Analysis. See the link below for a sample CofA:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/D1501-BULKSLBW1517.pdf役に立ちましたか?
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