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Merck

CWA-1080

Sigma-Aldrich

Anti-Human CD178/FasL (NOK-1) ColorWheel® Dye-Ready mAb

greener alternative

for use with ColorWheel® Dyes (Required, (Sold Separately))

別名:

Tumor necrosis factor ligand superfamily member 6

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.41
製品CWA-1080は現在お客様の国では販売されていません。 製品に関するお問い合わせはこちら(テクニカルサービス)

由来生物

mouse

品質水準

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

NOK-1, monoclonal

製品種目

ColorWheel®

フォーム

lyophilized

分子量

calculated mol wt 31.49 kDa

精製方法

using Protein A

化学種の反応性

human

包装

antibody small pack of 25 μL

環境により配慮した代替製品の特徴

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

テクニック

flow cytometry: suitable

アイソタイプ

IgG1κ

エピトープ配列

C-terminal

タンパク質IDアクセッション番号

UniProtアクセッション番号

適合性

for use with ColorWheel® Dyes (Required, (Sold Separately))

環境により配慮した代替製品カテゴリ

輸送温度

ambient

保管温度

2-8°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... FASLG(356)

詳細

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

特異性

Clone NOK-1 is a mouse monoclonal antibody that detects CD178 (FasL).

免疫原

L5178Y mouse T lymphoma cells expressing recombinant human CD178.

アプリケーション

Quality Control Testing

Evaluated by Flow Cytometry in HEK293 cells transfected with CD178.

Staining of one million transiently CD178-transfected HEK 293 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1080, Anti-Human CD178/FasL (NOK-1) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel® Antibody-ready Allophycocyanin (APC) Dye.

Evaluated by Flow Cytometry in NK cells.

Flow Cytometry Analysis: Staining of one million Human NK cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1080, Anti-Human CD178 (NOK-1) ColorWheel® Dye-Ready mAb and Cat. No. CWD-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWD-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

適合性

Requires ColorWheel® Dye (Sold Separately)

ターゲットの説明

Tumor necrosis factor ligand superfamily member 6 (UniProt: P48023; also known as Apoptosis antigen ligand, APTL, CD95 ligand, CD95-L, Fas antigen ligand, Fas ligand, FasL, CD178) is encoded by the FASLG (also known as APT1LG1, CD95L, FASL, TNFSF6) gene (Gene ID: 356) in human. Fas Ligand is a single-pass, homotrimeric, type II membrane protein with a cytoplasmic domain (aa 1-80), a transmembrane domain (aa 81-102), and an extracellular domain (aa 103-281). The membrane-bound FasL (mFasL) undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10, which produces an N-terminal fragment that lacks the receptor-binding extracellular domain. This ADAM10-processed FasL remnant form is still membrane anchored and further processed by signal peptide peptidase like 2A (SPPL2A) that liberates the FasL intracellular domain (FasL ICD). mFasL can also undergo proteolytic cleavage to produce a truncated soluble form (sFasL; aa 130-281). However, sFasL does not activate Fas, and it competes with the membrane bound FasL to reduce its cytotoxic activity. sFasL can bind to Fas, but does not induce its oligomerization, thereby preventing activation of the proapoptotic signaling pathway by the membrane bound FasL. FasL can also undergo N-glycosylation that enhances its apoptotic activity. Two isoforms of FasL have been described that are produced by alternative splicing. (Ref.: Volpe, E., et al. (2016). Front. Immunol. 7; 382; Schneider, P., et al. (1997). J. Biol. Chem. 272(30); 18827-18833).

物理的形状

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

再構成

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

法的情報

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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製品番号
詳細
価格

保管分類コード

11 - Combustible Solids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

CWA-1080-25UL:


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試験成績書(COA)

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質問

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アクティブなフィルタ

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)