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製品名
U2OS RFP-TUBA1B,
由来生物
human female bone (Source Disease: Osteosarcoma)
OMIMアクセッション番号
保管温度
−196°C
遺伝子情報
human ... alpha tubulin 1B(10376)
詳細
U2OS RFP-TUBA1Bは、白人女性(15歳)から得られた骨肉腫、上皮細胞で、GFPタグ修飾されています。
This product was derived from ATCC Cat. No. HTB-96.
アプリケーション
This product is a human U2OS cell line in which the genomic TUBA1B gene has been endogenously tagged with a Red Fluorescent Protein (RFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which RFP is attached to the N-terminus of tubulin. Fluorescence imaging shows that the tubulin can polymerize to form the characteristic pattern of microtubules. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage
特徴および利点
染色体12q13.12にGFPタグを付加
The U2OS cells are adherent, with a doubling time of approx. 29 hours.
構成
U2OS RFP-TUBA1B 細胞
品質
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
調製ノート
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
法的情報
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
CLL1034-1VL:
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