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product name
U2OS BFP-LMNB1 RFP-ACTB,
由来生物
human female bone (Disease source: Osteosarcoma)
保管温度
−196°C
遺伝子情報
human ... ACTB(60) , LMNB1(4001)
詳細
This product was derived from ATCC Cat. No. HTB-96.
U2OS BFP-LMNB1 RFP-ACTB are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with BFP-tagged and RFP-tagged modifications.
U2OS BFP-LMNB1 RFP-ACTB are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with BFP-tagged and RFP-tagged modifications.
アプリケーション
This product is a human U2OS cell line in which the genomic LaminB1 gene has been endogenously tagged with a Blue Fluorescent Protein (BFP) and the genomic ACTB gene has been endogenously tagged with a Red Fluorescent Protein (RFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of a fusion protein in which BFP is attached to the N-terminus of LaminB1 and of a fusion protein in which RFP is attached to the N-terminus of actin. Fluorescence imaging shows the characteristic LaminB1 nuclear expression and that the actin can polymerize to form the characteristic pattern of actin filaments. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage
特徴および利点
LMNB1 is BFP-tagged on chromosome 5q23.3-q31.1 and ACTB is RFP-tagged on chromosome 7p22.1.
U2OS cells are adherent, with a doubling time of approx. 29 hours.
U2OS cells are adherent, with a doubling time of approx. 29 hours.
構成
U2OS BFP-LMNB1 RFP-ACTB cells
品質
テスト項目:マイコプラズマ、無菌性、凍結後生存能の試験、細胞株同定のための短末端反復(STR)分析、細胞株の種確認のためのPCRアッセイ。
調製ノート
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
法的情報
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
CLL1038-1VL:
CLL1038-BULK:
CLL1038-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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