EMT6/AR10.0

96042326, mouse unknown/unspecified, Epithelial-like

• UNSPSCコード: 41106514

特性

• product name: EMT6/AR10.0, 96042326
• 由来生物: mouse unknown/unspecified
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse mammary, drug-resistant

細胞株の説明

The drug resistant cell line EMT6/AR10.0 was derived from the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344) by continuous growth in increasing concentrations of doxorubicin. It was reported to show reduced intracellular drug accumulation and hyperexpression of P-glycoprotein. It is recommended to culture the cells without drug after resuscitation until the first passage.

アプリケーション

Drug-resistance studies

培地

MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA)+ 10.0μg/ml doxorubicin + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5%CO₂; 37ºC; resuscitate in drug-free media, add doxorubicin after first passage

その他情報

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