L132 (HeLa derivative)

89111004, human cervix, Epithelial

• UNSPSCコード: 41106514

特性

• product name: L132 (HeLa derivative), 89111004
• 由来生物: human cervix
• 成長モード: Adherent
• 核型: 2n = 46
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human cervix carcinoma

細胞株の説明

Originally derived from a human embryonic lung. Used for primary isolation of enteroviruses and also found to be sensitive to hepatitis virus. The line shows HeLa markers. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

アプリケーション

Isolation of enteroviruses

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. The cells grow in clumps.

その他情報

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