MCF7 Cell Line human

86012803, human breast (adenocarcinoma), Epithelial-like

• 別名: IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells, ssMCF-7 Cells
• UNSPSCコード: 41106514
• eCl@ss: 32190102

特性

• product name: MCF7 Cell Line human, from human breast(adenocarcinoma), 86012803
• 由来生物: human breast (adenocarcinoma)
• 成長モード: Adherent
• 核型: 2n = 46, hypertriploid to hypotetraploid
• 形態: Epithelial-like
• 製品: Not specified
• 受容体: Reported to express the oestrogen and progesterone receptor
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian breast adenocarcinoma

細胞株の説明

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

アプリケーション

Tumourigenicity and virus studies: Type B and C viruses

DNAプロファイル

STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

培地

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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