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CHO-K1 Cell Line from hamster

85051005, hamster ovary, Epithelial

別名:

CHO cell clone K1 Cells, CHOK1 Cells

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About This Item

UNSPSCコード:
41106514
現在、価格および在庫状況を閲覧できません。

製品名

CHO-K1 Cell Line from hamster, 85051005, from hamster ovary

由来生物

hamster ovary

成長モード

Adherent

核型

Hypodiploid, modal no. 20

形態

Epithelial

製品

Not specified

受容体

Not specified

テクニック

cell culture | mammalian: suitable

輸送温度

dry ice

保管温度

−196°C

細胞株の由来

Hamster Chinese ovary

細胞株の説明

A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.

アプリケーション

CHO-K1 cell line from hamster has been used to study the effect of plasmid pCM01 induction on the association of bacteria with CHO-K1 cells by the gentamicin protection assay. It has also been cultured with or without lactate to measure time-series for cell proliferation.[1][2]
Nutritional and gene expression studies

培地

Ham′s F12+ 2mM Glutamine+ 10% Foetal Bovine Serum FBS / FCS.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25%Trypsin or Trypsin/EDTA; 5% CO2; 37°C.

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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資料

Cell Lines for Recombinant Protein Expression

Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines.

質問

1–9/9 質問  

  1. What is the approximate cells/cm2 of a 100% Confluent flask?

    1 回答

    1. A general guideline for the approximate number of cells per square centimeter in a 100% confluent flask is 1 x 10^5 cells/cm². For more detailed information, refer to the table on page 4 of the following brochure: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/318/066/corning_cc_guide.pdf

      役に立ちましたか？

  2. Is the cell like deficient of DHFR gene?

    1 回答

    1. CHO-K1 are dihydrofolate reductase (DHFR) positive. There are other mutants made from CHO-K1 that are DHFR deficient. Please see the links below to review these possible options:

      CHO/dhFr-
      https://www.sigmaaldrich.com/product/sigma/cb_94060607

      CHO/dhFr-AC-free
      https://www.sigmaaldrich.com/product/sigma/cb_05011002

      CHOZN® ZFN-Modified CHO
      https://www.sigmaaldrich.com/product/sigma/chodhfr

      役に立ちましたか？

  3. What passage are my Cell Line cells?

    1 回答

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

      役に立ちましたか？

  4. Are the Cell Line cells tested for viral pathogens?

    1 回答

    1. HPA Cultures does not perform any viral testing on the cell lines.

      役に立ちましたか？

  5. How many Cell Line cells are in the vial?

    1 回答

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

      役に立ちましたか？

  6. How do I handle Cell Line frozen cells upon arrival?

    1 回答

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

      役に立ちましたか？

  7. Are my Cell Line cells mycoplasma tested?

    1 回答

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

      役に立ちましたか？

  8. What medium should I use when I thaw the Cell Line cells?

    1 回答

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

      役に立ちましたか？

  9. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 回答

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

      役に立ちましたか？

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