B95-8

85011419, cotton-top tamarin monkey blood, Lymphoblast/Fibroblast

• UNSPSCコード: 41106514

特性

• product name: B95-8, from cotton-top tamarin monkey blood, 85011419, peripheral blood lymphocyte, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• 由来生物: cotton-top tamarin monkey blood
• 成長モード: Suspension
• 核型: Not specified
• 形態: Lymphoblast/Fibroblast
• 製品: Epstein Barr Virus (EBV)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Cotton-top Tamarin Monkey peripheral blood lymphocyte

細胞株の説明

Derived from a cotton-top tamarin (Saguinus oedipus). Releases high titres of transforming Epstein Bar virus (EBV). Thus it provides a source of EBV to establish continuous lymphocytic lines from human donors. The cells should be handled under laboratory containment level 2.

In some instances the cell line B95-8 has been described as derived from ′marmoset′; however, this is not correct. B95-8 was derived from a cotton-top tamarin (Saguinas oedipus); this has been confirmed by DNA profiling.

培地

RPMI 1640 (R0883)+ 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml. May need to use 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended that this cell line be frozen at no less than 8x1000,000 cells per ampoule.

その他情報

HPA Culture Collections由来でSIGMAによって供給される培養物は研究の目的のみに使用できます。商業目的での細胞株の使用に関する質問は、その細胞株のデポジターに照会されます。一部の細胞株には、細胞株の供給の前に受給予定者によって物質移動契約書への記入を完了させるという要求など、さらなる特別な公開条件があります。詳しくはTerms & Conditions of Supplyをご覧ください。