RCP

31626, rat brain, Endothelial

• UNSPSCコード: 41106514

特性

• product name: RCP, 00031626
• 由来生物: rat brain
• 成長モード: Adherent
• 核型: Not specified
• 形態: Endothelial
• 製品: Not specified
• 受容体: Vasopressin V1a, Angiotensin II, Endothelin 1, Bradykinin, Serotonin
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat Choroid Plexus

細胞株の説明

The choroid plexus of adult Wistar Rats were dissected from the fourth ventricle to give a primary culture. This primary culture was then immortalized by transfection with the TSori minus adenovirus (a replication deficient adenovirus containing SV40 large T antigen). Sub-cloning of the immortalised cells was performed by selecting for clones which exhibited calcium ion release in response to vasopressin, angiotensin, bradykinin, serotonin or endothelin stimulation. The sub-clone which exhibited the largest response to all five hormones was selected and sub-cultured on to become RCP.

アプリケーション

Toxicology, cellular signalling and pharmacology studies

培地

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 0.1% Epidermal Growth Factor(EGF) (E9644), 1% Insulin–Transferrin–Selenium(ITS) (I3146)

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

その他情報

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