C9282

コール酸ナトリウム 水和物

BioReagent, suitable for cell culture

• 別名: 3α,7α,12α-トリヒドロキシ-5β-コラン-24-酸 ナトリウム塩, コール酸 ナトリウム塩
• 実験式（ヒル表記法）: C₂₄H₃₉NaO₅ · xH₂O
• CAS番号: 206986-87-0
• 分子量: 430.55 (anhydrous basis)
• Beilstein: 3582354
• EC Number: 206-643-5
• MDL番号: MFCD00150749
• UNSPSCコード: 12161902
• PubChem Substance ID: 24893147
• NACRES: NA.75

特性

• 由来生物: bovine bile; ovine bile
• 詳細: anionic
• 製品種目: BioReagent
• アッセイ: ≥99.0% (HPLC)
• フォーム: powder
• 分子量: micellar avg mol wt 900-1300
• 凝集数: 2-3
• テクニック: cell culture | mammalian: suitable
• CMC: 9-15 mM (20-25°C)
• 溶解性: water: 100 mg/mL, clear, colorless to light yellow
• HLB: 18
• SMILES記法: O.[Na+].C[C@H](CCC([O-])=O)C1CCC2C3[C@H](O)CC4C[C@H](O)CC[C@]4(C)C3C[C@H](O)[C@]12C
• InChI: 1S/C24H40O5.Na.H2O/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26;;/h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29);;1H2/q;+1;/p-1/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-;;/m1../s1
• InChI Key: MUVVIYFKOVLQHL-RCVKHMDESA-M

詳細

詳細

Cholate is a primary bile acid[1] produced from cholesterol in the liver.[2] It is an anionic detergent synthesized from cholesterol in the presence of sterol 12α-hydroxylase (CYP8B1).[3]

アプリケーション

Cholate is a bile acid produced in liver during cholesterol breakdown. Cholate downregulates cholesterol-7-α-hydroxylase (rate-limiting step in bile acid synthesis). Cholate, an anionic detergent, is used alone and in combination with urea to extract and reconstitute membrane proteins and protein complexes.

Sodium cholate hydrate has been used:

• as a bile acid to induce bile acid-responsive (BEAR) transcription system[4],
• as a component of Campylobacter defined broth (CDB) to test its effect on stress response in Campylobacter jejuni{52
• to test its sensitivity towards the bile acid sensor[5]

生物化学的／生理学的作用

Reduction in 12-α hydroxylase activity leads to low levels of cholic acid in liver disease.[2]

安全性情報

• 危険有害性情報: H412
• 注意書き: P273 - P501
• 危険有害性の分類: Aquatic Chronic 3
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)