C-20111

Keratinocyte Growth Medium 2

Kit including Basal Medium and SupplementPack, 500 ml

• 別名: Primary Cell Culture Media
• UNSPSCコード: 12352207
• NACRES: NA.71

特性

• 形状: liquid
• 包装: pkg of 500 ml
• テクニック: cell culture | mammalian: suitable
• 輸送温度: wet ice

詳細

詳細

Keratinocyte Growth Medium 2 Kit

アプリケーション

Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells. The medium is optimized for primary human cells but can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.Recommended for:Normal Human Epidermal Keratinocytes (NHEK), juvenile foreskinNormal Human Epidermal Keratinocytes (NHEK), adult donor

構成

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

品質

All lots of PromoCell Keratinocyte Growth Medium 2 are subjected to comprehensive quality control tests using primary human keratinocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested keratinocytes. Approved inhouse lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

調製ノート

Final supplement concentrations after addition to the basal medium:Bovine Pituitary Extract: 0.004 ml / mlEpidermal Growth Factor (recombinant human): 0.125 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 0.33 µg / mlEpinephrine: 0.39 µg / mlTransferrin (recombinant human): 10 µg / mlCaCl2: 0.06 mMNote: CaCl2 is provided as separate stock solution. This allows users to adjust the CaCl2 concentration individually. To ensure the maximal growth performance of normal human Keratinocytes, PromoCell recommends adding the entire 60 μlCaCl2 stock solution, which results in final 0.06 mM CaCl2 concentration. In case a higher CaCl2 concentration is required (e.g. differentiation experiments), a 0.5 M CaCl2-Solution can be ordered separately (C-34006).

保管および安定性

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix, the CaCl2 solution, or the SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

継代と培養方法

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安全性情報

• ピクトグラム: GHS02,GHS05
• シグナルワード: Danger
• 危険有害性情報: H225,H290,H319
• 注意書き: P210 - P233 - P234 - P240 - P241 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1
• 保管分類コード: 3 - Flammable liquids