T17917

トリシン

≥99% (titration), reagent grade, crystalline

• 別名: N-[トリス(ヒドロキシメチル)メチル]グリシン
• 化学式: (HOCH₂)₃CNHCH₂CO₂H
• CAS番号: 5704-04-1
• 分子量: 179.17
• Beilstein: 1937804
• MDL番号: MFCD00004277
• UNSPSCコード: 12161700
• NACRES: NA.25

特性

• 由来生物: synthetic
• 品質水準: 200
• グレード: reagent grade
• アッセイ: ≥99% (titration)
• フォーム: crystalline
• 保管条件: (Tightly closed. Dry)
• 不純物: <0.5% water
• 色: white
• 有効pH範囲: 7.4-8.8
• pKa (25 °C): 8.1
• mp: 187 °C
• 溶解性: water: 0.1 M, clear, colorless
• 微量陽イオン: Pb: ≤5 ppm
• 保管温度: room temp
• SMILES記法: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N

詳細

詳細

Tricine, a zwitterionic biological buffer, is commonly employed in research applications such as enzyme assays, electrophoresis, cell biology, and biochemical studies. Its increased negative charge compared to glycine ensures rapid migration during electrophoresis, facilitating efficient separation of low-molecular-weight proteins in gels with lower acrylamide percentages. Moreover, Tricine may also be used in cryopreservation media, aiding in the preservation of tissues and organs.

Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.

アプリケーション

低分子量ペプチド分離用のバッファー成分です。

Tricine is intended to use for:

• Electrophoresis To separate low-weight peptides and proteins
• Crystallization of proteins of varying pH
• Resuspension of cell pellets
• Enzyme assays
• Tissue culture

特徴および利点

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7.4 - 8.8 with a pKa of 8.1 (25 °C)

その他情報

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安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable