product line
pHast Pack™
form
powder
technique(s)
nucleic acid detection: suitable
pH
8.3
suitability
suitable for gel electrophoresis
foreign activity
DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected
SMILES string
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key
OSBLTNPMIGYQGY-UHFFFAOYSA-N
General description
Application
TBE保存濃縮液を1倍TBEランニングバッファーにまで希釈すると、バッファーには89 mMのトリス-ホウ酸と2 mMのEDTAが含まれるようになり、pHは8.3です。 5倍または10倍の保存濃縮液も、PAGEゲルを作製するためにアクリルアミド/ビス-アクリルアミド保存溶液に添加できます。 最大の分解能を得るには、5 V/cm(装置の電極間の距離)未満の印加電圧を推奨します。
- DNA agarose gel electrophoresis (fragment <1500 bp)
- Native and non-denaturing RNA agarose gel electrophoresis (fragment <1500 bp)
- Denaturing gradient gel electrophoresis
- Northern and Southern blotting
- DNA automated sequencing gel
- Pulsed-field gel electrophoresis (PFGE) (applied voltages of less than 5 V/cm are recommended for maximum resolution)
Features and Benefits
- Ready-to-use buffer
- No measuring and pH adjusting needed
- Save time and minimize effort
- Minimize chemical exposure to toxic chemicals
- Biological tests: free of DNase, RNase, Protease, and Nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
Packaging
Preparation Note
Other Notes
Legal Information
1 of 1
当該品目 | |||
|---|---|---|---|
| description pH 8.3, pHast Pack™, powder | description pH 8.3, pHast Pack™, powder | description 10× concentrate, BioReagent, Molecular Biology, non-sterile; 0.2 μm filtered | description pHast Pack™, powder |
| technique(s) nucleic acid detection: suitable | technique(s) nucleic acid detection: suitable | technique(s) - | technique(s) - |
| product line pHast Pack™ | product line pHast Pack™ | product line BioReagent | product line pHast Pack™ |
| form powder | form powder | form solution | form powder |
| pH 8.3 | pH 8.3 | pH - | pH 8.1-8.5(range for the Tris Borate ETDA buffer prior to blending with agarose) |
| suitability suitable for gel electrophoresis | suitability suitable for gel electrophoresis, suitable for gel electrophoresis (after dilution to working concentration) | suitability suitable for gel electrophoresis (after dilution to working concentration) | suitability - |
| foreign activity Protease, none detected, DNAse, none detected, Nickase, none detected, RNAse, none detected | foreign activity DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected | foreign activity Protease, none detected, RNAse, none detected | foreign activity NICKase, none detected, Exonuclease, none detected, Endonuclease , RNase, none detected, DNase, none detected |
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注意喚起語
Danger
ハザードコード
Hazard Classifications
Repr. 1B
保管分類
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
引火点 (F)
Not applicable
引火点(°C)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
第一種指定化学物質
prtr
名称等を表示すべき危険物及び有害物
ishl_indicated
名称等を通知すべき危険物及び有害物
ishl_notified
PPB009-20PAK:4548173361888
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