glucose, 20 mM magnesium chloride, 10 mM magnesium sulfate, 10 mM potassium chloride, 2.5 mM sodium chloride, 10 mM tryptone, 20 g/L yeast extract, 5 g/L
テクニック
microbiological culture: suitable
最終pH
7.0±0.2 (25 °C)
アプリケーション
food and beverages life science and biopharma microbiology
保管温度
2-8°C
適合性
nonselective for Escherichia coli nonselective for coliforms
アプリケーション
SOC Broth is a sterile solution for the recovery of electroporated Escherichia coli cells.[1]
調製ノート
Immediately after electroporation add 1 ml sterile SOC Medium to the transformed cells and incubate for 30-60 minutes at 37°C with a moderate shaking .
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法的情報
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth