SCT152
Firefly/Renilla Dual Luciferase Assay
Flash-type dual luciferase assay that allows measurement of both Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity.
別名:
Firefly Luciferase Assay, Dual Luciferase Assay, Bioluminescent Assay
ログイン組織・契約価格を表示する
すべての画像(2)
About This Item
UNSPSCコード:
12352207
NACRES:
NA.54
おすすめの製品
テクニック
cell based assay: suitable
検出方法
chemiluminescent
輸送温度
ambient
詳細
Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays.
The Firefly/Renilla Dual Luciferase Assay allows measurement of both Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity. Firefly luciferase activity is measured first, then Renilla Luciferase Assay Buffer 2.0 is added to simultaneously quench firefly luciferase activity and measure Renilla luciferase activity. Renilla Luciferase Assay Buffer 2.0 quenches the firefly luciferase activity to the level of untransfected cells, allowing sequential measurement of firefly and Renilla luciferase activity in the same sample. This is a flash-type assay that requires luminescence to be measured immediately after adding the detection reagents to the luciferase sample. Firefly signal decays over the course of about 12 minutes, while Renilla signal decays over the course of about 2 minutes, although this may vary depending on enzyme levels.
The Firefly/Renilla Dual Luciferase Assay allows measurement of both Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity. Firefly luciferase activity is measured first, then Renilla Luciferase Assay Buffer 2.0 is added to simultaneously quench firefly luciferase activity and measure Renilla luciferase activity. Renilla Luciferase Assay Buffer 2.0 quenches the firefly luciferase activity to the level of untransfected cells, allowing sequential measurement of firefly and Renilla luciferase activity in the same sample. This is a flash-type assay that requires luminescence to be measured immediately after adding the detection reagents to the luciferase sample. Firefly signal decays over the course of about 12 minutes, while Renilla signal decays over the course of about 2 minutes, although this may vary depending on enzyme levels.
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Firefly Luciferase Assay
Flash-type dual luciferase assay that allows measurement of both Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity.
Research Sub Category
Live Cell Dye
Live Cell Dye
構成
1) 1X Passive Luciferase Lysis Buffer 2.0 (CS224514): 15 mL
2) Firefly Luciferase Assay Buffer 2.0 (CS224591): 15 mL
3) D-Luciferin (CS224551): 3 X 1 mg
4) Renilla Luciferase Assay Buffer 2.0 (CS224580): 15 mL
5) Aquaphile Coelenterazine (CS224581): 3 X 200 µg
2) Firefly Luciferase Assay Buffer 2.0 (CS224591): 15 mL
3) D-Luciferin (CS224551): 3 X 1 mg
4) Renilla Luciferase Assay Buffer 2.0 (CS224580): 15 mL
5) Aquaphile Coelenterazine (CS224581): 3 X 200 µg
物理的形状
Liquid
保管および安定性
Store Firefly/Renilla Dual Luciferase Assay at -80°C. Firefly and Renilla Assay Buffers are stable at -80°C for at least six months from date of receipt. Other components are stable at -20°C or below for at least six months from date of receipt. Kit components and stock solutions of D-Luciferin and Aquaphile Coelenterazine in water are stable to at least 5 freeze/thaw cycles.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SCT152:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Xinyu Tang et al.
Bioengineered, 12(1), 4643-4653 (2021-07-30)
Non-small cell lung cancer (NSCLC) is one of the main causes of death in the world. To improve the diagnostic level and find new biological targets，GSE datasets were selected from GEO databaseto analyze the differential expression genes and construct ceRNA
Joseph M Chan et al.
Cancer cell, 39(11), 1479-1496 (2021-10-16)
Small cell lung cancer (SCLC) is an aggressive malignancy that includes subtypes defined by differential expression of ASCL1, NEUROD1, and POU2F3 (SCLC-A, -N, and -P, respectively). To define the heterogeneity of tumors and their associated microenvironments across subtypes, we sequenced
資料
Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）