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Merck

SCM013

Sigma-Aldrich

NDiff Neuro-27 Medium Supplement (100x)

NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants & other factors that has been developed for the in vitro propagation & maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium.

別名:

NDiff Medium Supplement, Neuro-27 Medium Supplement

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About This Item

UNSPSCコード:
12352207
eCl@ss:
32160801
NACRES:
NA.75

品質水準

形状

liquid

メーカー/製品名

Chemicon®
Millipore

テクニック

cell culture | stem cell: suitable

入力

sample type: mouse embryonic stem cell(s)

輸送温度

dry ice

詳細

NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants and other factors that has been specifically developed for the in vitro propagation and maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium (Nichols J, 2006). This product can also be used to differentiate murine ES cells into post-mitotic neurons particularly via monolayer differentiation (Ying QL, 2003).

物理的形状

NDiff Neuro-27 Medium Supplement is supplied as an aqueous solution. Optimal working concentration of the medium supplement must be determined for specific uses and cell types. In most instances, a 1:40-1:200 dilution is appropriate. Typically, Neuro-27 is diluted 1:100 for self renewal and neural differentiation of mouse ES cells.

保管および安定性

Maintain at -20 °C until expiration date. Protect from light. Once thawed, aliquot unused portion and store at -20 °C until future use. Once added to the cell culture medium, the product is stable at 4 °C for four weeks.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

SCM013:


試験成績書(COA)

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Defined conditions for neural commitment and differentiation
Ying, Qi-Long and Smith, Austin G
Test, 365, 327-341 (2003)
Qi-Long Ying et al.
Nature biotechnology, 21(2), 183-186 (2003-01-14)
Mouse embryonic stem (ES) cells are competent for production of all fetal and adult cell types. However, the utility of ES cells as a developmental model or as a source of defined cell populations for pharmaceutical screening or transplantation is
Jennifer Nichols et al.
Methods in molecular biology (Clifton, N.J.), 329, 91-98 (2006-07-19)
The availability of murine embryonic stem (ES) cells has revolutionized the study of mammalian development and disease. We recently developed a culture medium that has enabled us to identify the essential signaling pathways required for maintenance of pluripotency in vitro.

資料

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

オルガノイド培養に関するFAQ3D細胞培養プロトコルに従ったオルガノイドの培養・分析方法をご覧ください。ヒントとコツ。

プロトコル

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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