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由来生物
rat
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
ER-TR4, monoclonal
精製方法
affinity chromatography
化学種の反応性
mouse
包装
antibody small pack of 100 μg
テクニック
immunofluorescence: suitable
immunohistochemistry: suitable
アイソタイプ
IgMκ
エピトープ配列
Unknown
タンパク質IDアクセッション番号
UniProtアクセッション番号
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
詳細
The thymus is considered as the primary lymphoid organ that is responsible for the generation and maturation of T cells. Thymic epithelial cells (TECs) account for the majority of thymic stromal components. These cells can be divided into cortical and medullary thymic epithelial cells based on their localization within the thymus. TECs have dual germ layer origins, with cortical cells arising from ectoderm and medullary cells arising from the endoderm. In an embryonic thymus, cortical cells are the dominant population, while medullary cells account for most of the TECs in the adult thymus. Cortical thymic epithelial cells are essential for the positive selection of T cells, whereas the medullary thymic epithelial cells (mTECS) play a role in inducing negative selection of highly reactive T cells that are required for establishing central self-tolerance. mTECs participate in self-tolerance by eliminating self-reactive T cells. Cortical cells that support positive selection also support post-selection maturation of single-positive thymocytes. They provide cytokines, such as IL-7 and SCF, to promote early thymic progenitors (ETP) cells and Delta-like ligand 4 (DLL4)-mediated Notch signaling to induce the differentiation of ETPs towards the T lineage. DLL4 is reported to be expressed at high levels in cortical cells, but not in medullary cells. Clone ER-TR4 reacts with epithelial-reticular cells in the cortex of murine thymus. (Ref.: Wang, HX., et al. (2020). Front. Immunol. 10; 3099; Van Vliet, E., et al. (1984). Eur. J. Immunol. 14(6); 524-529; Cordier, AC., et al. (1980). Am. J Anat. 157(3); 227-263).
特異性
Clone ER-TR4 is a rat monoclonal antibody that detects cortical stromal cells.
免疫原
Thymic stromal cells isolated from C3H mice.
アプリケーション
Quality Control Testing
Evaluated by Immunohistochemistry in mouse thymus tissue sections.
Immunohistochemistry Applications: A 1:50 dilution of this antibody detected Cortical stromal cells in mouse thymus tissue sections.
Tested applications
Immunofluorescence Analysis: A representative lot detected Cortical stromal cells in Immunofluorescence applications (Tanaka, Y., et al., (2007). Blood. 110(1):107-15).
Immunohistochemistry Applications: A representative lot detected Cortical stromal cells in Immunohistochemistry applications (Van Vliet, E., et al., (1984). Eur J Immunol. 14(6):524-9; Scheijen, B., et al., (2004). J Biol Chem. 279(11):10476-83; Dooley, J., et al., (2006). J Immunol. 176(11):6484-90; Tanaka, Y., et al., (2007). Blood. 110(1):107-15; Moretti, F.A., et al., (2018). Elife. 7:e35816; Hollander, G.A., et al. (1995). Nature. 373(6512):350-3).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Immunohistochemistry in mouse thymus tissue sections.
Immunohistochemistry Applications: A 1:50 dilution of this antibody detected Cortical stromal cells in mouse thymus tissue sections.
Tested applications
Immunofluorescence Analysis: A representative lot detected Cortical stromal cells in Immunofluorescence applications (Tanaka, Y., et al., (2007). Blood. 110(1):107-15).
Immunohistochemistry Applications: A representative lot detected Cortical stromal cells in Immunohistochemistry applications (Van Vliet, E., et al., (1984). Eur J Immunol. 14(6):524-9; Scheijen, B., et al., (2004). J Biol Chem. 279(11):10476-83; Dooley, J., et al., (2006). J Immunol. 176(11):6484-90; Tanaka, Y., et al., (2007). Blood. 110(1):107-15; Moretti, F.A., et al., (2018). Elife. 7:e35816; Hollander, G.A., et al. (1995). Nature. 373(6512):350-3).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
物理的形状
Purified rat monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
再構成
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Recommended storage: +2°C to +8°C.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABF2816-100UG:
MABF2816-25UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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