MABE224

Anti-ZFX Antibody, clone 3E8.1

clone 3E8.1, from mouse

• 別名: Zinc finger X-chromosomal protein
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 3E8.1, monoclonal
• 交差性: human
• テクニック: immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_001171555
• UniProtアクセッション番号: P17010
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ZFX(7543)

詳細

詳細

ZFX, also known as Zinc finger X-chromosomal protein, and encoded by the gene name ZFX, is probable transcriptional activator. This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined.

免疫原

GST-tagged recombinant protein corresponding to human ZFX.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
核受容体

Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected ZFX HeLa cells.

This Anti-ZFX antibody is validated for use in WB, IC for the detection of ZFX.

品質

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZFX in 200 µg of HeLa nuclear extract.

ターゲットの説明

~130, ~90, ~59 kDa observed. Three bands (~130 kDa, ~91 kDa and ~59 kDa) corresponding to ZFX were detected. The ~130 kDa band is nuclear, while the ~91 kDa band was found in the cytoplasmic (Zhu, Z., et al. (2013). J Neurooncol. 112:17-25).

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2