Cranin (dystroglycan), extracellular site, near the C-terminus of the alpha-subunit. The expression and localization of cranin varies considerably according to the cells physiologic state and differentiaion state. MAB371 recognizes the same peptide region as MAB373 but a different epitope.
免疫原
Epitope: aa572-604 of human sequence
Synthetic peptide corresponding to a.a. 572-604 of human cranin.
アプリケーション
Research Category 細胞骨格
細胞骨格
Research Sub Category 細胞骨格
ECMタンパク質
Immunohistochemistry on tissue fixed briefly with 0.5% glutaraldehyde, 2% PLP fixation, methanol or acetone. Prolonged fixation must be avoided. Traditional formalin is not recommended. Suggested working dilution 1:1-1:50.
Immunocytochemistry on neuron, astrocyte, smooth muscle, fibroblast and epithelial cell lines.
ELISA
Not suggested for use in Western blot.
Optimal working dilutions must be determined by end user.
This Anti-Cranin Antibody, aa572-604 of human sequence, clone 6C1 is validated for use in ELISA, IC, IH for the detection of Cranin.
物理的形状
Ascites Liquid.
保管および安定性
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The Journal of cell biology, 153(4), 811-822 (2001-05-16)
Fibroblast growth factors and receptors are intimately connected to the extracellular matrix by their affinity to heparan sulfate proteoglycans. They mediate multiple processes during embryonic development and adult life. In this study, embryonic stem cell-derived embryoid bodies were used to