IP02

Protein A Agarose Suspension

Name: Protein A Agarose Suspension
Brand: MM

Protein A Agarose Suspension designed for immunoprecipitation applications

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UNSPSCコード:

41116133

NACRES:

NA.56

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フォーム

slurry (Liquid)

含みます

≤0.1% sodium azide as preservative

メーカー／製品名

Calbiochem^®

保管条件

do not freeze

輸送温度

wet ice

保管温度

2-8°C

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当該品目	IP05	IP04	P1925
Millipore IP02 Protein A Agarose Suspension	Millipore IP05 Protein G Plus/Protein A Agarose Suspension	Millipore IP04 Protein G Plus-Agarose Suspension	Millipore P1925 プロテインA–アガロースマクロビーズ
form slurry (Liquid)	form slurry (Liquid)	form slurry (Liquid)	form aqueous suspension
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains -
manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename -
storage condition do not freeze	storage condition do not freeze	storage condition do not freeze	storage condition -
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in -

詳細

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG_2a and IgG_2b and rabbit IgG.

Protein A conjugated to agarose.

物理的形状

In PBS.

その他情報

Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG_2a or IgG_2b monoclonal antibodies. Mouse IgG₁ or IgG₃ and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Toxicity: Standard Handling (A)

保管分類コード

11 - Combustible Solids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

IP02-1.5ML:

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA）を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

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p38β Mitogen-Activated Protein Kinase Modulates Its Own Basal Activity by Autophosphorylation of the Activating Residue Thr180 and the Inhibitory Residues Thr241 and Ser261.

Jonah Beenstock et al.

Molecular and cellular biology, 36(10), 1540-1554 (2016-03-16)

Many enzymes are self-regulated and can either inhibit or enhance their own catalytic activity. Enzymes that do both are extremely rare. Many protein kinases autoactivate by autophosphorylating specific sites at their activation loop and are inactivated by phosphatases. Although mitogen-activated

GemC1 governs multiciliogenesis through direct interaction with and transcriptional regulation of p73.

Maria-Eleni Lalioti et al.

Journal of cell science, 132(11) (2019-04-28)

A distinct combination of transcription factors elicits the acquisition of a specific fate and the initiation of a differentiation program. Multiciliated cells (MCCs) are a specialized type of epithelial cells that possess dozens of motile cilia on their apical surface.

Protein arginine methyltransferase expression, localization, and activity during disuse-induced skeletal muscle plasticity.

Derek W Stouth et al.

American journal of physiology. Cell physiology, 314(2), C177-C190 (2017-11-03)

Protein arginine methyltransferase 1 (PRMT1), PRMT4, and PRMT5 catalyze the methylation of arginine residues on target proteins. Previous work suggests that these enzymes regulate skeletal muscle plasticity. However, the function of PRMTs during disuse-induced muscle remodeling is unknown. The purpose

RANK signaling increases after anti-HER2 therapy contributing to the emergence of resistance in HER2-positive breast cancer.

Adrián Sanz-Moreno et al.

Breast cancer research : BCR, 23(1), 42-42 (2021-04-01)

Around 15-20% of primary breast cancers are characterized by HER2 protein overexpression and/or HER2 gene amplification. Despite the successful development of anti-HER2 drugs, intrinsic and acquired resistance represents a major hurdle. This study was performed to analyze the RANK pathway

Differential binding modes of the bromodomains of CREB-binding protein (CBP) and p300 with acetylated MyoD.

Lanlan Wei et al.

Biochemical and biophysical research communications, 368(2), 279-284 (2008-01-29)

The recruitment of the bromodomains of CREB-binding protein (CBP) and p300 by the acetylated myogenic transcription factor MyoD was previously shown to be critical for the enhanced MyoD transcriptional activity following acetylation at its Lys99 and Lys102 positions. However, the

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