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Merck

HPLX1-115SP-PX

Millipore

MILLIPLEX® PLEXpedition Screening Premixed Kits (80-plex & 115-plex)

別名:

Human screening kit, Human screening panel, Luminex human screening immunoassay, Luminex human screening panel, Millipore screening kit, Millipore screening panel, Multiplex screening kit, Multiplex screening panel

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About This Item

UNSPSCコード:
12161503
NACRES:
NA.84

詳細

96 well

品質水準

化学種の反応性

human

メーカー/製品名

Milliplex®

パラメーター

25 μL sample volume (per well)

assay range

intra-assay cv: ≤15%
inter-assay cv: ≤20%
(≤25% for PDGF-AA)

テクニック

multiplexing: suitable using : Suitable

入力

cell culture supernatant(s)
serum
plasma

検出方法

fluorometric (Luminex® xMAP®)

輸送温度

wet ice

保管温度

2-8°C

詳細

The MILLIPLEX® PLEXpedition Screening Panel is a configurable multiplex immunoassay kit developed to simultaneously screen up to 115 targets including cytokines, chemokines, growth factors, matrix metalloproteinases (MMPs), and biomarkers of bone health, metabolism, and cardiovascular disease. Two Luminex® instruments, the xMAP® INTELLIFLEX and the FLEXMAP 3D®, can analyze all 115 bead regions with comparable sensitivity and sample correlation while the MAGPIX® and Luminex 200 instruments can detect specific subsets of 50 and 80 analytes, respectively. As a discovery biomarker tool this panel is provided in a matrix-free format to enable analyte detection in multiple sample types.

特異性

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel, with the following exceptions:

  • GIP and ENA-78 have low cross-reactivity with IL-23 (≤15%) and GRO (≤5%) recombinant proteins, respectively.
  • IL-12 (p40) has moderate, but expected, cross-reactivity with the recombinant proteins for IL-12 (p70) and IL-23 which both contain the p40 subunit.
  • MMP-3 may exhibit significant cross-reactivity with MMP-10 recombinant proteins due to high sequence homology, however, analysis demonstrated independent and differentiated measurement of MMP-3 and MMP-10 in biological samples.

アプリケーション

The MILLIPLEX® PLEXpedition Magnetic Bead Screening Panel is to be used for the simultaneous quantification of up to 115 analytes in your selected sample type. (NOTE: Instrument requirements vary based on analyte selection.) This is an overnight assay and requires 25 µL per well of neat or diluted sample.

  • MAGPIX®/Luminex 200/FLEXMAP 3D®/xMAP® INTELLIFLEX required for any or all of the following 50 analytes: EGF, FGF-2, Fractalkine, G-CSF, Ghrelin (Active), GIP, Glucagon, GM-CSF, Granzyme B, GROα, HGF, IFNα2, IFNγ, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-18, IL-1Ra, IL-1α, IL-1β, IL-2, IL-22, IL-23, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, Leptin, MCP-1, MIG, MIP-1α, MIP-1β, OPN, PDGF-AA, PDGF-BB, Perforin, RANTES, sCD40L, SCF, sFAS, sFASL, TGFα, TNFα, TRAIL, VEGF-A
  • Luminex 200/FLEXMAP 3D®/xMAP® INTELLIFLEX required for any or all of the following 80 analytes: ACTH, BDNF, C-Peptide, EGF, Eotaxin, FABP4, FGF-2, FLT3 Ligand, Fractalkine, G-CSF, Ghrelin (Active), GIP, GLP-1 Total, Glucagon, GM-CSF, Granzyme A, Granzyme B, GROα, HGF, IFNα2, IFNγ, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-18, IL-1Ra, IL-1α, IL-1β, IL-2, IL-21, IL-22, IL-23, IL-3, IL-33, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, Insulin, IP-10, I-TAC, Leptin, MCP-1, MCP-3, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-3α, MMP-1, MMP-2, MMP-7, MMP-9, OPG, OPN, PDGF-AA, PDGF-BB, Perforin, PlGF, PP, PYY, RANTES, sCD40L, SCF, sFAS, sFASL, TARC, TGFα, TNFα, TNFβ, TRAIL, Troponin I, VEGF-A
  • FLEXMAP 3D®/xMAP® INTELLIFLEX required for any or all of the following 115 analytes: ACTH, Amylin (Active), BCA-1, BDNF, BNP, CK-MB, C-Peptide, DKK1, EGF, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, Erythropoeitin (EPO), FABP3, FABP4, FGF-2, FGF-21, FGF-23, FLT3 Ligand, Fractalkine, G-CSF, Ghrelin (Active), GIP, GLP-1 Total, Glucagon, GM-CSF, GPC-2, Granzyme A, Granzyme B, GROα, HB-EGF, HGF, I-309, IFNα2, IFNγ, IL-10, IL-11, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-18, IL-1Ra, IL-1α, IL-1β, IL-2, IL-21, IL-22, IL-23, IL-3, IL-33, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, Insulin, IP-10, I-TAC, Leptin, LIF, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-1Ϭ, MIP-3α, MIP-3β, MMP-1, MMP-10, MMP-12, MMP-2, MMP-3, MMP-7, MMP-9, Myostatin , NGF, NTproBNP, OPG, OPN, Osteocalcin, Osteonectin, PDGF-AA, PDGF-BB, Perforin, PlGF, PP, PYY, RANTES, sCD40L, SCF, SDF-1, sFAS, sFASL, SOST, TARC, TGFα, TNFα, TNFβ, TPO, TRAIL, Troponin I, TSLP, VEGF-A, VEGF-C, VEGF-D

特徴および利点

This is a configurable kits. Design your multiplex kit by choosing available analytes within this panel based on your instrument.

法的情報

FLEXMAP 3D system is a registered trademark of Luminex Corp
Luminex is a registered trademark of Luminex Corp
Luminex 200 system is a trademark of Luminex Corp
MAGPIX system is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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関連コンテンツ

Simplify protein biomarker discovery with the MILLIPLEX® PLEXpedition 115-Plex Multiplex Screening Panel. Effortlessly screen cytokines and other markers with Luminex® technology.

Discover the benefits of MILLIPLEX® multiplex assays, based on Luminex® xMAP® multiplex assay technology, that provide consistent, high-quality results and see how these multiplex biomarker Luminex® assays are being used to advance research.

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